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PMID: 1745 Published · ppublish English Journal Article

Neuronal properties of hybrid neuroblastoma X sympathetic ganglion cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 12 ·1975-12-00 ·Pages 4923-7

Greene LA, Shain W, Chalazonitis A, Breakfield X, Minna J, Coon HG, Nirenberg M

Abstract

Clonal mouse neuroblastoma cells without tyrosine 3-monooxygenase [EC 1.14.16.2; tyrosine hydroxylase; L-tyrosine, tetrahydropteridine:oxygen oxidoreductase (3-hydroxylating)] activity were fused with normal cells from embryonic mouse sympathetic ganglia. One of the 37 hybrid cell lines obtained possesses high tyrosine 3-monooxygenase activity and synthesizes dopamine. These cells also have excitable membranes and generate action potentials in response to electrical stimuli. Thus hybrid cells, generated by fusion of neuroblastoma cells with normal cells from the nervous system, can acquire neural properties not found with the parental neuroblastoma cells.

MeSH Terms
Action Potentials Animals Bucladesine/pharmacology Cell Line Chromosomes Ganglia, Autonomic/drug effects,enzymology,physiology Glucose-6-Phosphate Isomerase/metabolism Hybrid Cells/drug effects,enzymology,physiology Hypoxanthine Phosphoribosyltransferase/metabolism Karyotyping Mice Neuroblastoma/enzymology,pathology Neurons/physiology Tyrosine 3-Monooxygenase/metabolism
Chemicals
Bucladesine Tyrosine 3-Monooxygenase Hypoxanthine Phosphoribosyltransferase Glucose-6-Phosphate Isomerase
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Greene L A
Shain W
Chalazonitis A
Breakfield X
Minna J
Coon H G
Nirenberg M
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22 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.12.4923
Published
1975-12-00
Pages
4923-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC388845
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.12.4923 PMC Full Text View journal details
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