Home LiteratureArticle Details
PMID: 17526914 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

The first genetic and comparative map of white lupin (Lupinus albus L.): identification of QTLs for anthracnose resistance and flowering time, and a locus for alkaloid content.

DNA research : an international journal for rapid publication of reports on genes and genomes ·Vol. 14 ·No. 2 ·2007-04-00 ·Pages 59-70

Phan HT, Ellwood SR, Adhikari K, Nelson MN, Oliver RP

Abstract

We report the first genetic linkage map of white lupin (Lupinus albus L.). An F8 recombinant inbred line population developed from Kiev mutant x P27174 was mapped with 220 amplified fragment length polymorphism and 105 gene-based markers. The genetic map consists of 28 main linkage groups (LGs) that varied in length from 22.7 cM to 246.5 cM and spanned a total length of 2951 cM. There were seven additional pairs and 15 unlinked markers, and 12.8% of markers showed segregation distortion at P < 0.05. Syntenic relationships between Medicago truncatula and L. albus were complex. Forty-five orthologous markers that mapped between M. truncatula and L. albus identified 17 small syntenic blocks, and each M. truncatula chromosome aligned to between one and six syntenic blocks in L. albus. Genetic mapping of three important traits: anthracnose resistance, flowering time, and alkaloid content allowed loci governing these traits to be defined. Two quantitative trait loci (QTLs) with significant effects were identified for anthracnose resistance on LG4 and LG17, and two QTLs were detected for flowering time on the top of LG1 and LG3. Alkaloid content was mapped as a Mendelian trait to LG11.

MeSH Terms
Alkaloids/metabolism Biological Evolution Chromosome Mapping Flowers/growth & development Lupinus/genetics,growth & development,metabolism,microbiology Medicago truncatula/genetics Plant Diseases/genetics,microbiology Quantitative Trait Loci Species Specificity
Chemicals
Alkaloids
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Phan Huyen T T
Australian Centre for Necrotrophic Fungal Pathogens, SABC, Department of Health Sciences, Murdoch University, Murdoch 6150, Australia.
Ellwood Simon R
Adhikari Kedar
Nelson Matthew N
Oliver Richard P
References (39)
39 references, click to expand
  1. Genome relationships: the grass model in current research.
    Plant Cell. 2000 May;12(5):637-46 PMID: 10810140
  2. Through a genome, darkly: comparative analysis of plant chromosomal DNA.
    Plant Mol Biol. 2002 Jan;48(1-2):5-20 PMID: 11860213
  3. Lupin kernel fibre-enriched foods beneficially modify serum lipids in men.
    Eur J Clin Nutr. 2005 Mar;59(3):325-33 PMID: 15508014
  4. Cluster roots--an underground adaptation for survival in extreme environments.
    Trends Plant Sci. 2002 Apr;7(4):162-7 PMID: 11950612
  5. Map Manager QTX, cross-platform software for genetic mapping.
    Mamm Genome. 2001 Dec;12(12):930-2 PMID: 11707780
  6. AFLP: a new technique for DNA fingerprinting.
    Nucleic Acids Res. 1995 Nov 11;23(21):4407-14 PMID: 7501463
  7. Phylogeny and genomic organization of the TIR and non-tIR NBS-LRR resistance gene family in Medicago truncatula.
    Mol Plant Microbe Interact. 2002 Jun;15(6):529-39 PMID: 12059101
  8. Comparative mapping between Medicago sativa and Pisum sativum.
    Mol Genet Genomics. 2004 Oct;272(3):235-46 PMID: 15340836
  9. Metabolic changes associated with cluster root development in white lupin (Lupinus albus L.): relationship between organic acid excretion, sucrose metabolism and energy status.
    Planta. 2001 Aug;213(4):534-42 PMID: 11556785
  10. An informative linkage map of soybean reveals QTLs for flowering time, leaflet morphology and regions of segregation distortion.
    DNA Res. 2001 Apr 27;8(2):61-72 PMID: 11347903
  11. Mapping quantitative trait loci in chickpea associated with time to flowering and resistance to Didymella rabiei the causal agent of Ascochyta blight.
    Theor Appl Genet. 2006 Nov;113(7):1357-69 PMID: 17016689
  12. Cross-species amplification of Medicago truncatula microsatellites across three major pulse crops.
    Theor Appl Genet. 2005 May;110(7):1210-7 PMID: 15806349
  13. Estimating genome conservation between crop and model legume species.
    Proc Natl Acad Sci U S A. 2004 Oct 26;101(43):15289-94 PMID: 15489274
  14. Sweet lupins in human nutrition.
    World Rev Nutr Diet. 1995;77:75-88 PMID: 7732701
  15. An intraspecific linkage map of the chickpea ( Cicer arietinum L.) genome based on sequence tagged microsatellite site and resistance gene analog markers.
    Theor Appl Genet. 2003 May;106(8):1447-56 PMID: 12750788
  16. Recombinant inbred lines for genetic mapping in tomato.
    Theor Appl Genet. 1995 Mar;90(3-4):542-8 PMID: 24173949
  17. Medicago truncatula EST-SSRs reveal cross-species genetic markers for Medicago spp.
    Theor Appl Genet. 2004 Feb;108(3):414-22 PMID: 13679975
  18. Comparison of a Brassica oleracea genetic map with the genome of Arabidopsis thaliana.
    Genetics. 2003 May;164(1):359-72 PMID: 12750346
  19. Development of nuclear gene-derived molecular markers linked to legume genetic maps.
    Mol Genet Genomics. 2006 Jul;276(1):56-70 PMID: 16642337
  20. Comparative physical mapping reveals features of microsynteny between Glycine max, Medicago truncatula, and Arabidopsis thaliana.
    Genome. 2004 Feb;47(1):141-55 PMID: 15060611
  21. Mapping polygenes.
    Annu Rev Genet. 1993;27:205-33 PMID: 8122902
  22. SSR analysis of the Medicago truncatula SARDI core collection reveals substantial diversity and unusual genotype dispersal throughout the Mediterranean basin.
    Theor Appl Genet. 2006 Mar;112(5):977-83 PMID: 16402186
  23. Chromosomal regions associated with segregation distortion of molecular markers in F2, backcross, doubled haploid, and recombinant inbred populations in rice (Oryza sativa L.).
    Mol Gen Genet. 1997 Feb 20;253(5):535-45 PMID: 9065686
  24. Comparative genomics enabled the isolation of the R3a late blight resistance gene in potato.
    Plant J. 2005 Apr;42(2):251-61 PMID: 15807786
  25. Construction of a genetic linkage map using MFLP and identification of molecular markers linked to domestication genes in narrow-leafed lupin (Lupinus angustifolius L.).
    Cell Mol Biol Lett. 2005;10(2):331-44 PMID: 16010297
  26. Mapping genetic factors controlling pollen viability in an interspecific cross in Helianthus sect. Helianthus.
    Theor Appl Genet. 1995 Dec;91(8):1195-202 PMID: 24170046
  27. Functional mapping in pea, as an aid to the candidate gene selection and for investigating synteny with the model legume Medicago truncatula.
    Theor Appl Genet. 2006 Apr;112(6):1024-41 PMID: 16416153
  28. The first gene-based map of Lupinus angustifolius L.-location of domestication genes and conserved synteny with Medicago truncatula.
    Theor Appl Genet. 2006 Jul;113(2):225-38 PMID: 16791689
  29. Nuclear DNA content variation and species relationships in the genus Lupinus (Fabaceae).
    Ann Bot. 2003 Sep;92(3):349-55 PMID: 12853281
  30. Cereal genome evolution. Grasses, line up and form a circle.
    Curr Biol. 1995 Jul 1;5(7):737-9 PMID: 7583118
  31. Extensive macrosynteny between Medicago truncatula and Lens culinaris ssp. culinaris.
    Theor Appl Genet. 2007 Feb;114(3):549-58 PMID: 17119911
  32. Bridging model and crop legumes through comparative genomics.
    Plant Physiol. 2005 Apr;137(4):1189-96 PMID: 15824281
  33. Comparative genome mapping in Brassica.
    Genetics. 1996 Dec;144(4):1903-10 PMID: 8978073
  34. Comparative mapping and marker-assisted selection in Rosaceae fruit crops.
    Proc Natl Acad Sci U S A. 2004 Jun 29;101(26):9891-6 PMID: 15159547
  35. Phosphorus deficiency in Lupinus albus. Altered lateral root development and enhanced expression of phosphoenolpyruvate carboxylase.
    Plant Physiol. 1996 Sep;112(1):31-41 PMID: 8819319
  36. Genetic mapping of an ancient translocation in the genus Lens.
    Theor Appl Genet. 1987 Apr;73(6):883-92 PMID: 24241299
  37. Identification of perennial ryegrass (Lolium perenne (L.)) and meadow fescue (Festuca pratensis (Huds.)) candidate orthologous sequences to the rice Hd1(Se1) and barley HvCO1 CONSTANS-like genes through comparative mapping and microsynteny.
    New Phytol. 2005 Jul;167(1):239-47 PMID: 15948846
  38. A sequence-based genetic map of Medicago truncatula and comparison of marker colinearity with M. sativa.
    Genetics. 2004 Mar;166(3):1463-502 PMID: 15082563
  39. High-resolution mapping of the barley leaf rust resistance gene Rph5 using barley expressed sequence tags (ESTs) and synteny with rice.
    Theor Appl Genet. 2005 Nov;111(8):1651-60 PMID: 16195886
Article Info
Journal
DNA research : an international journal for rapid publication of reports on genes and genomes
Abbr.
DNA Res
ISSN
1340-2838
DOI
10.1093/dnares/dsm009
Published
2007-04-00
Epub
2007-00-26
Pages
59-70
Language
English
Region
England
NLM ID
9423827
PMCID
PMC2779896
Subset
IM
External Links
PubMed source DOI: 10.1093/dnares/dsm009 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2