Home LiteratureArticle Details
PMID: 17536181 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Functional validation of a novel isoform of Na+/H+ antiporter from Pennisetum glaucum for enhancing salinity tolerance in rice.

Journal of biosciences ·Vol. 32 ·No. 3 ·2007-04-00 ·Pages 621-8

Verma D, Singla-Pareek SL, Rajagopal D, Reddy MK, Sopory SK

Abstract

Salt stress is an environmental factor that severely impairs plant growth and productivity. We have cloned a novel isoform of a vacuolar Na+/H+ antiporter from Pennisetum glaucum (PgNHX1) that contains 5 transmembrane domains in contrast to AtNHX1 and OsNHX1 which have 9 transmembrane domains. Recently we have shown that PgNHX1 could confer high level of salinity tolerance when overexpressed in Brassica juncea. Here,we report the functional validation of this antiporter in crop plant rice. Overexpression of PgNHX1 conferred high level of salinity tolerance in rice. Transgenic rice plants overexpressing PgNHX1 developed more extensive root system and completed their life cycle by setting flowers and seeds in the presence of 150 mM NaCl. Our data demonstrate the potential of PgNHX1 for imparting enhanced salt tolerance capabilities to salt-sensitive crop plants for growing in high saline areas.

MeSH Terms
Cloning, Molecular Dose-Response Relationship, Drug Gene Expression Regulation, Plant Germination Oryza/drug effects,genetics,growth & development,metabolism Pennisetum/genetics Plant Leaves/drug effects,metabolism Plants, Genetically Modified Protein Isoforms/chemistry,genetics,metabolism Reproducibility of Results Sodium Chloride/pharmacology Sodium-Hydrogen Exchangers/chemistry,genetics,metabolism
Chemicals
Protein Isoforms Sodium-Hydrogen Exchangers Sodium Chloride
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Verma Dheeraj
Plant Molecular Biology, International Center for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 10067, India.
Singla-Pareek Sneh L
Rajagopal Divya
Reddy M K
Sopory S K
References (29)
29 references, click to expand
  1. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS.
    Plant Physiol. 1949 Jan;24(1):1-15 PMID: 16654194
  2. Overexpression of a plasma membrane Na+/H+ antiporter gene improves salt tolerance in Arabidopsis thaliana.
    Nat Biotechnol. 2003 Jan;21(1):81-5 PMID: 12469134
  3. Genes and salt tolerance: bringing them together.
    New Phytol. 2005 Sep;167(3):645-63 PMID: 16101905
  4. Na+/myo-inositol symporters and Na+/H+-antiport in Mesembryanthemum crystallinum.
    Plant J. 2000 Nov;24(4):511-22 PMID: 11115132
  5. Isolation and characterization of a Na+/H+ antiporter gene from the halophyte Atriplex gmelini.
    Plant Mol Biol. 2001 May;46(1):35-42 PMID: 11437248
  6. Transgenic salt-tolerant tomato plants accumulate salt in foliage but not in fruit.
    Nat Biotechnol. 2001 Aug;19(8):765-8 PMID: 11479571
  7. Introduction of a Na+/H+ antiporter gene from Atriplex gmelini confers salt tolerance to rice.
    FEBS Lett. 2002 Dec 18;532(3):279-82 PMID: 12482579
  8. Plant productivity and environment.
    Science. 1982 Oct 29;218(4571):443-8 PMID: 17808529
  9. Engineering salt-tolerant Brassica plants: characterization of yield and seed oil quality in transgenic plants with increased vacuolar sodium accumulation.
    Proc Natl Acad Sci U S A. 2001 Oct 23;98(22):12832-6 PMID: 11606781
  10. Plant salt tolerance.
    Trends Plant Sci. 2001 Feb;6(2):66-71 PMID: 11173290
  11. Antisense suppression of phospholipase D alpha retards abscisic acid- and ethylene-promoted senescence of postharvest Arabidopsis leaves.
    Plant Cell. 1997 Dec;9(12):2183-96 PMID: 9437863
  12. A rice quantitative trait locus for salt tolerance encodes a sodium transporter.
    Nat Genet. 2005 Oct;37(10):1141-6 PMID: 16155566
  13. Arabidopsis H+-PPase AVP1 regulates auxin-mediated organ development.
    Science. 2005 Oct 7;310(5745):121-5 PMID: 16210544
  14. Rapid increase of vacuolar volume in response to salt stress.
    Planta. 2003 Jan;216(3):397-402 PMID: 12520330
  15. The Arabidopsis thaliana salt tolerance gene SOS1 encodes a putative Na+/H+ antiporter.
    Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6896-901 PMID: 10823923
  16. Identification of a 170-kDa protein associated with the vacuolar Na+/H+ antiport of Beta vulgaris.
    Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11177-81 PMID: 1662387
  17. Vacuolar cation/H+ exchange, ion homeostasis, and leaf development are altered in a T-DNA insertional mutant of AtNHX1, the Arabidopsis vacuolar Na+/H+ antiporter.
    Plant J. 2003 Oct;36(2):229-39 PMID: 14535887
  18. Genes encoding the vacuolar Na+/H+ exchanger and flower coloration.
    Plant Cell Physiol. 2001 May;42(5):451-61 PMID: 11382810
  19. Trehalose accumulation in rice plants confers high tolerance levels to different abiotic stresses.
    Proc Natl Acad Sci U S A. 2002 Dec 10;99(25):15898-903 PMID: 12456878
  20. Characterization of a novel Na+/H+ antiporter gene InNHX2 and comparison of InNHX2 with InNHX1, which is responsible for blue flower coloration by increasing the vacuolar pH in the Japanese morning glory.
    Plant Cell Physiol. 2005 Feb;46(2):259-67 PMID: 15695437
  21. Salt tolerance conferred by overexpression of a vacuolar Na+/H+ antiport in Arabidopsis.
    Science. 1999 Aug 20;285(5431):1256-8 PMID: 10455050
  22. Function, intracellular localization and the importance in salt tolerance of a vacuolar Na(+)/H(+) antiporter from rice.
    Plant Cell Physiol. 2004 Feb;45(2):146-59 PMID: 14988485
  23. Genetic engineering of the glyoxalase pathway in tobacco leads to enhanced salinity tolerance.
    Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):14672-7 PMID: 14638937
  24. Molecular cloning and expression of the Na+/H+ exchanger gene in Oryza sativa.
    Biochim Biophys Acta. 1999 Jul 7;1446(1-2):149-55 PMID: 10395929
  25. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
    Anal Biochem. 1987 Apr;162(1):156-9 PMID: 2440339
  26. Identification and characterization of a NaCl-inducible vacuolar Na+/H+ antiporter in Beta vulgaris.
    Physiol Plant. 2002 Oct;116(2):206-212 PMID: 12354197
  27. The cotton GhNHX1 gene encoding a novel putative tonoplast Na(+)/H(+) antiporter plays an important role in salt stress.
    Plant Cell Physiol. 2004 May;45(5):600-7 PMID: 15169942
  28. Comparative molecular analysis of Na+/H+ exchangers: a unified model for Na+/H+ antiport?
    FEBS Lett. 1998 Mar 6;424(1-2):1-5 PMID: 9537504
  29. PLANT CELLULAR AND MOLECULAR RESPONSES TO HIGH SALINITY.
    Annu Rev Plant Physiol Plant Mol Biol. 2000 Jun;51:463-499 PMID: 15012199
Article Info
Journal
Journal of biosciences
Abbr.
J Biosci
ISSN
0250-5991
DOI
10.1007/s12038-007-0061-9
Published
2007-04-00
Pages
621-8
Language
English
Region
India
NLM ID
8100809
Subset
IM
External Links
PubMed source DOI: 10.1007/s12038-007-0061-9 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2