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PMID: 17569867 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S.

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.

Science (New York, N.Y.) ·Vol. 316 ·No. 5831 ·2007-06-15 ·Pages 1625-8

Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE

Abstract

Genome sequences for most metazoans and plants are incomplete because of the presence of repeated DNA in the heterochromatin. The heterochromatic regions of Drosophila melanogaster contain 20 million bases (Mb) of sequence amenable to mapping, sequence assembly, and finishing. We describe the generation of 15 Mb of finished or improved heterochromatic sequence with the use of available clone resources and assembly methods. We also constructed a bacterial artificial chromosome-based physical map that spans 13 Mb of the pericentromeric heterochromatin and a cytogenetic map that positions 11 Mb in specific chromosomal locations. We have approached a complete assembly and mapping of the nonsatellite component of Drosophila heterochromatin. The strategy we describe is also applicable to generating substantially more information about heterochromatin in other species, including humans.

MeSH Terms
Animals Chromosome Mapping Chromosomes, Artificial, Bacterial Contig Mapping Drosophila melanogaster/genetics Genome Heterochromatin/genetics In Situ Hybridization, Fluorescence Physical Chromosome Mapping Sequence Analysis, DNA
Chemicals
Heterochromatin
Authors & Affiliations
14 authors, click to expand affiliations / ORCID
Hoskins Roger A
Department of Genome and Computational Biology, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
Carlson Joseph W
Kennedy Cameron
Acevedo David
Evans-Holm Martha
Frise Erwin
Wan Kenneth H
Park Soo
Mendez-Lago Maria
Rossi Fabrizio
Villasante Alfredo
Dimitri Patrizio
Karpen Gary H
Celniker Susan E
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Article Info
Journal
Science (New York, N.Y.)
Abbr.
Science
ISSN
1095-9203
DOI
10.1126/science.1139816
Published
2007-06-15
Pages
1625-8
Language
English
Region
United States
NLM ID
0404511
PMCID
PMC2825053
Subset
IM
Grants
NHGRI NIH HHS · P50-HG00750 · United States
NIGMS NIH HHS · R01 GM066272 · United States
NHGRI NIH HHS · R01 HG00747 · United States
NHGRI NIH HHS · R01 HG000747 · United States
NHGRI NIH HHS · R01 HG000747-16 · United States
External Links
PubMed source DOI: 10.1126/science.1139816 PMC Full Text View journal details
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