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PMID: 17577400 Published · epublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

An early response regulatory cluster induced by low temperature and hydrogen peroxide in seedlings of chilling-tolerant japonica rice.

BMC genomics ·Vol. 8 ·2007-06-18 ·Pages 175

Cheng C, Yun KY, Ressom HW, Mohanty B, Bajic VB, Jia Y, Yun SJ, de los Reyes BG

Abstract

Plants respond to low temperature through an intricately coordinated transcriptional network. The CBF/DREB-regulated network of genes has been shown to play a prominent role in freeze-tolerance of Arabidopsis through the process of cold acclimation (CA). Recent evidence also showed that the CBF/DREB regulon is not unique to CA but evolutionarily conserved between chilling-insensitive (temperate) and chilling-sensitive (warm-season) plants. In this study, the wide contrast in chilling sensitivity between indica and japonica rice was used as model to identify other regulatory clusters by integrative analysis of promoter architecture (ab initio) and gene expression profiles. Transcriptome analysis in chilling tolerant japonica rice identified a subset of 121 'early response' genes that were upregulated during the initial 24 hours at 10 degrees C. Among this group were four transcription factors including ROS-bZIP1 and another larger sub-group with a common feature of having as1/ocs-like elements in their promoters. Cold-induction of ROS-bZIP1 preceded the induction of as1/ocs-like element-containing genes and they were also induced by exogenous H2O2 at ambient temperature. Coordinated expression patterns and similar promoter architectures among the 'early response' genes suggest that they belong to a potential regulon (ROS-bZIP-as1/ocs regulatory module) that responds to elevated levels of ROS during chilling stress. Cultivar-specific expression signatures of the candidate genes indicate a positive correlation between the activity of the putative regulon and genotypic variation in chilling tolerance. A hypothetical model of an ROS-mediated regulon (ROS-bZIP-as1/ocs) triggered by chilling stress was assembled in rice. Based on the current results, it appears that this regulon is independent of ABA and CBF/DREB, and that its activation has an important contribution in configuring the rapid responses of rice seedlings to chilling stress.

MeSH Terms
Base Sequence Cold Temperature Gene Expression Profiling Gene Expression Regulation, Plant Genes, Plant Genomics Hydrogen Peroxide/pharmacology Molecular Sequence Data Multigene Family Oryza/genetics,physiology Plant Physiological Phenomena Plant Proteins/metabolism Reactive Oxygen Species Temperature Transcription Factors/metabolism
Chemicals
Plant Proteins Reactive Oxygen Species Transcription Factors Hydrogen Peroxide
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Cheng Chen
Department of Biological Sciences, University of Maine, Orono, ME 04469, USA. [email protected] <[email protected]>
Yun Kil-Young
Ressom Habtom W
Mohanty Bijayalaxmi
Bajic Vladimir B
Jia Yulin
Yun Song Joong
de los Reyes Benildo G
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Article Info
Journal
BMC genomics
Abbr.
BMC Genomics
ISSN
1471-2164
DOI
10.1186/1471-2164-8-175
Email
[email protected]
Published
2007-06-18
Epub
2007-00-18
Pages
175
Language
English
Region
England
NLM ID
100965258
PMCID
PMC1925099
Subset
IM
External Links
PubMed source DOI: 10.1186/1471-2164-8-175 PMC Full Text View journal details
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