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PMID: 15383661 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

A prominent role for the CBF cold response pathway in configuring the low-temperature metabolome of Arabidopsis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 101 ·No. 42 ·2004-10-19 ·Pages 15243-8

Cook D, Fowler S, Fiehn O, Thomashow MF

Abstract

The Arabidopsis CBF cold response pathway has a central role in cold acclimation, the process whereby plants increase in freezing tolerance in response to low nonfreezing temperatures. Here we examined the changes that occur in the Arabidopsis metabolome in response to low temperature and assessed the role of the CBF cold response pathway in bringing about these modifications. Of 434 metabolites monitored by GC-time-of-flight MS, 325 (75%) were found to increase in Arabidopsis Wassilewskija-2 (Ws-2) plants in response to low temperature. Of these 325 metabolites, 256 (79%) also increased in nonacclimated Ws-2 plants in response to overexpression of C-repeat/dehydration responsive element-binding factor (CBF)3. Extensive cold-induced changes also occurred in the metabolome of Arabidopsis Cape Verde Islands-1 (Cvi-1) plants, which were found to be less freezing tolerant than Ws-2 plants. However, low-temperature-induced expression of CBF1, CBF2, CBF3, and CBF-targeted genes was much lower in Cvi-1 than in Ws-2 plants, and the low-temperature metabolome of Cvi-1 plants was depleted in metabolites affected by CBF3 overexpression. Taken together, the results indicate that the metabolome of Arabidopsis is extensively reconfigured in response to low temperature, and that the CBF cold response pathway has a prominent role in this process.

MeSH Terms
Acclimatization/genetics,physiology Arabidopsis/genetics,metabolism Arabidopsis Proteins/genetics,metabolism Cold Climate DNA-Binding Proteins/genetics,metabolism Gene Expression Regulation, Plant Genome, Plant Models, Biological Regulon Trans-Activators/genetics,metabolism
Chemicals
Arabidopsis Proteins DNA-Binding Proteins Trans-Activators
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Cook Daniel
Michigan State University-Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, MI 48824, USA.
Fowler Sarah
Fiehn Oliver
Thomashow Michael F
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.0406069101
Published
2004-10-19
Epub
2004-00-21
Pages
15243-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC524070
Subset
IM
Corrections
CommentIn
PMID: 15479761
External Links
PubMed source DOI: 10.1073/pnas.0406069101 PMC Full Text View journal details
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