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PMID: 17588529 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Fluorescence probe partitioning between Lo/Ld phases in lipid membranes.

Biochimica et biophysica acta ·Vol. 1768 ·No. 9 ·2007-09-00 ·Pages 2182-94

Baumgart T, Hunt G, Farkas ER, Webb WW, Feigenson GW

Abstract

Fluorescence microscopy imaging is an important technique for studying lipid membranes and is increasingly being used for examining lipid bilayer membranes, especially those showing macroscopic coexisting domains. Lipid phase coexistence is a phenomenon of potential biological significance. The identification of lipid membrane heterogeneity by fluorescence microscopy relies on membrane markers with well-defined partitioning behavior. While the partitioning of fluorophores between gel and liquid-disordered phases has been extensively characterized, the same is not true for coexisting liquid phases. We have used fluorescence microscopy imaging to examine a large variety of lipid membrane markers for their liquid phase partitioning in membranes with various lipid compositions. Most fluorescent lipid analogs are found to partition strongly into the liquid-disordered (L(d)) phase. In contrast, some fluorescent polycyclic aromatic hydrocarbons with a flat ring system were found to partition equally, but others partition preferentially into liquid-ordered (L(o)) phases. We have found these fluorescent markers effective for identification of coexisting macroscopic membrane phases in ternary lipid systems composed of phospholipids and cholesterol.

MeSH Terms
Lipid Bilayers/chemistry Liposomes/chemistry Membrane Fluidity Microscopy, Fluorescence/methods Molecular Conformation Molecular Probe Techniques Phase Transition Phospholipids/chemistry
Chemicals
Lipid Bilayers Liposomes Phospholipids
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Baumgart Tobias
School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA.
Hunt Geoff
Farkas Elaine R
Webb Watt W
Feigenson Gerald W
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Article Info
Journal
Biochimica et biophysica acta
Abbr.
Biochim Biophys Acta
ISSN
0006-3002
DOI
10.1016/j.bbamem.2007.05.012
Published
2007-09-00
Epub
2007-00-21
Pages
2182-94
Language
English
Region
Netherlands
NLM ID
0217513
PMCID
PMC2702987
Subset
IM
Grants
NIBIB NIH HHS · P41 EB001976 · United States
NIBIB NIH HHS · P41 EB001976-17 · United States
NIBIB NIH HHS · 9 P41 EB001976 · United States
NIAID NIH HHS · AI18603 · United States
External Links
PubMed source DOI: 10.1016/j.bbamem.2007.05.012 PMC Full Text
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