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PMID: 176429 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Genomic complexities of murine leukemia and sarcoma, reticuloendotheliosis, and visna viruses.

Journal of virology ·Vol. 17 ·No. 2 ·1976-02-00 ·Pages 525-37

Beemon KL, Faras AJ, Hasse AT, Duesberg PH, Maisel JE

Abstract

The genetic complexities of several ribodeoxyviruses were measured by quantitative analysis of unique RNase T1-resistant oligonucleotides from 60-70S viral RNAs. Moloney murine leukemia virus was found to have an RNA complexity of 3.5 x 10(6) daltons, whereas Moloney murine sarcoma virus had a significantly smaller genome size of 2.3 x 10(6). Reticuleondotheliosis and visna virus RNAs had complexities of 3.9 x 10(6), respectively. Analysis of RNase A-resistant oligonucleotides of Rous sarcoma virus RNA gave a complexity of 3.6 x 10(6), similar to that previously obtained with RNase T1-resistant oligonucleotides. Since each of these viruses was found to have a unique sequence genomic complexity near the molecular weight of a single 30-40S viral RNA subunit, it was concluded that ribodeoxyvirus genomes are at least largely polyploid.

MeSH Terms
Base Sequence Gammaretrovirus/analysis Oligonucleotides/analysis RNA Viruses/analysis Reticuloendotheliosis virus/analysis Retroviridae/analysis Ribonucleases/metabolism Sarcoma Viruses, Murine/analysis Visna-maedi virus/analysis
Chemicals
Oligonucleotides Ribonucleases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Beemon K L
Faras A J
Hasse A T
Duesberg P H
Maisel J E
References (41)
41 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.17.2.525-537.1976
Published
1976-02-00
Pages
525-37
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC515444
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.17.2.525-537.1976 PMC Full Text View journal details
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