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PMID: 176432 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

High-titer replication of nondefective Sendai virus in MDBK cells.

Journal of virology ·Vol. 17 ·No. 2 ·1976-02-00 ·Pages 597-604

Famulari NG, Fleissner E

Abstract

Egg-grown Sendai virus was adapted to growth in a bovine kidney cell line (MDBK cells) by serial passage under defined conditions. The adapted virus contained only 50S RNA and was highly infectious for MDBK cells. Infection of these cells with a high multiplicity of adapted virus resulted in a yield of 10(8) MDBK-infectious units/ml by 18 h, accompanied by severe cytopathic changes in the host. Cell fusion did not occur. Examination of the proteins of the adapted virus revealed that despite the high infectivity of this virus for MDBK cells the virions contained considerable quantities of Fo, the precursor to the F glycoprotein that is responsible for cell fusion and high infectivity in other systems.

MeSH Terms
Adaptation, Biological Animals Cattle Cytopathogenic Effect, Viral Glycoproteins/analysis Kidney Parainfluenza Virus 1, Human/analysis,growth & development Peptides/analysis Protein Precursors/analysis RNA, Viral/analysis Viral Proteins/analysis
Chemicals
Glycoproteins Peptides Protein Precursors RNA, Viral Viral Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Famulari N G
Fleissner E
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20 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.17.2.597-604.1976
Published
1976-02-00
Pages
597-604
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC515450
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.17.2.597-604.1976 PMC Full Text View journal details
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