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PMID: 176631 Published · ppublish English Journal Article

A sedimentation study of the interaction of superhelical SV40 DNA with H1 histone.

Nucleic acids research ·Vol. 3 ·No. 2 ·1976-02-00 ·Pages 419-29

Böttger M, Scherneck S, Fenske H

Abstract

By moving boundary sedimentation it is shown that the interaction of H1 histone with superhelical circular SV40 DNA results in the formation of giant heterogeneous aggregates. The size of these aggregates grows with increasing H1 concentration. s20,w values of some 10 000 S were measured. As compared with open relaxed circular DNA a preferential interaction of superhelical DNA with H1 histone is observed, irrespective of the sign of the superhelical turns which was reversed by the addition to DNA of ethidium bromide. The addition to the H1 complexed aggregates of ethidium bromide effects a progressive breakdown of the aggregates. Furthermore, the superhelicity of DNA is not changed by the addition of small amounts of H1 histone.

MeSH Terms
Binding Sites DNA, Circular DNA, Viral Histones Macromolecular Substances Protein Binding Simian virus 40
Chemicals
DNA, Circular DNA, Viral Histones Macromolecular Substances
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Böttger M
Scherneck S
Fenske H
References (22)
22 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/3.2.419
Published
1976-02-00
Pages
419-29
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342912
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/3.2.419 PMC Full Text View journal details
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