A method for the selective extraction of histone fractions f2(a)1 and f2(a)2 from calf thymus deoxyribonucleoprotein at pH7.

The Biochemical journal ·Vol. 105 ·No. 2 ·1967-11-00 ·Pages 611-4

Johns EW

Abstract

1. A new method has been developed for the specific extraction of histone fraction f2(a) from calf thymus deoxyribonucleoprotein at pH7 by using a mixture of ethanol and guanidinium chloride. 2. Fraction f2(a) has been separated into the subfractions f2(a)1 and f2(a)2 by acetone precipitation from acid solution, and at pH7. 3. Modifications of existing electrophoretic methods are described that enable these fractions to be more easily characterized.

MeSH Terms

Acrylic Resins Amino Acids/analysis Animals Autoanalysis Cattle Chemical Precipitation DNA/analysis Electrophoresis Ethanol Gels Histones/analysis Hydrogen-Ion Concentration Methods Models, Chemical Nucleoproteins/analysis Solubility Solvents Starch Thymus Gland/analysis

Chemicals

Acrylic Resins Amino Acids Gels Histones Nucleoproteins Solvents Ethanol Starch DNA

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Johns E W

References (16)

16 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1050611

Published

1967-11-00

Pages

611-4

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1198352

Subset

External Links

PubMed source DOI: 10.1042/bj1050611 PMC Full Text View journal details

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A method for the selective extraction of histone fractions f2(a)1 and f2(a)2 from calf thymus deoxyribonucleoprotein at pH7.

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