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PMID: 6035526 Published · ppublish English Journal Article

The electrophoresis of histones in polyacrylamide gel and their quantitative determination.

The Biochemical journal ·Vol. 104 ·No. 1 ·1967-07-00 ·Pages 78-82

Johns EW

Abstract

1. A new method has been devised for the separation of the histone fractions of calf thymus by electrophoresis in polyacrylamide gel at pH2.4. 2. The fractions have been characterized by their relative mobilities with respect to a marker protein, bovine plasma albumin. 3. A method has been developed for the quantitative determination of the separated histone fractions by measuring the colour yields of dye-histone complexes formed in the gel.

MeSH Terms
Acrylic Resins Animals Cattle Electrophoresis Gels Histones/analysis In Vitro Techniques Thymus Gland
Chemicals
Acrylic Resins Gels Histones
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Johns E W
References (16)
16 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1040078
Published
1967-07-00
Pages
78-82
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1270547
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1040078 PMC Full Text View journal details
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