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PMID: 17674146 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Review

Regulating double-stranded DNA break repair towards crossover or non-crossover during mammalian meiosis.

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology ·Vol. 15 ·No. 5 ·2007-00-00 ·Pages 565-77

Baudat F, de Massy B

Abstract

During meiosis the programmed induction of DNA double-stranded breaks (DSB) leads to crossover (CO) and non-crossover products (NCO). One key role of CO is to connect homologs before metaphase I and thus to ensure the proper reductional segregation. This role implies an accurate regulation of CO frequency with the establishment of at least one CO per chromosome arm. Current major challenges are to understand how CO and NCO formation are regulated and what is the role of NCO. We present here the current knowledge about CO and NCO and their regulation in mammals. CO density varies widely along chromosomes and their distribution is not random as they are subject to positive interference. As documented in the mouse and human, a significant excess of DSB are generated relative to the number of CO. In fact, evidence has been obtained for the formation of NCO products, for regulation of the choice of DSB repair towards CO or NCO and for a CO specific pathway. We discuss the roles of Msh4, Msh5 and Sycp1 which affect DSB repair and probably not only the CO pathway. We suggest that, in mammals, the regulation of NCO differs from that described in Saccharomyces cerevisiae.

MeSH Terms
Animals Crossing Over, Genetic DNA Breaks, Double-Stranded DNA Repair/genetics,physiology Endodeoxyribonucleases Esterases/genetics,physiology Female Humans Male Meiosis/genetics,physiology Mice Models, Genetic Saccharomyces cerevisiae/genetics
Chemicals
Endodeoxyribonucleases Esterases meiotic recombination protein SPO11
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Baudat Frédéric
Institute of Human Genetics, UPR1142/CNRS, 141 rue de la Cardonille, 34396, Montpellier cedex 5, France.
de Massy Bernard
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Article Info
Journal
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology
Abbr.
Chromosome Res
ISSN
0967-3849
DOI
10.1007/s10577-007-1140-3
Published
2007-00-00
Pages
565-77
Language
English
Region
Netherlands
NLM ID
9313452
Subset
IM
External Links
PubMed source DOI: 10.1007/s10577-007-1140-3 View journal details
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