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PMID: 17699629 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

The role of fluctuations in tRNA selection by the ribosome.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 104 ·No. 34 ·2007-08-21 ·Pages 13661-5

Lee TH, Blanchard SC, Kim HD, Puglisi JD, Chu S

Abstract

The detailed mechanism of how the ribosome decodes protein sequence information with an abnormally high accuracy, after 40 years of study, remains elusive. A critical element in selecting correct transfer RNA (tRNA) transferring correct amino acid is "induced fit" between the ribosome and tRNA. By using single-molecule methods, the induced fit mechanism is shown to position favorably the correct tRNA after initial codon recognition. We provide evidence that this difference in positioning and thermal fluctuations constitutes the primary mechanism for the initial selection of tRNA. This work demonstrates thermal fluctuations playing a critical role in the substrate selection by an enzyme.

MeSH Terms

Fluorescence Resonance Energy Transfer Kinetics Models, Biological RNA, Transfer/genetics Ribosomes/genetics

Chemicals

RNA, Transfer

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Lee Tae-Hee

Departments of Physics and Applied Physics, Stanford University, Stanford, CA 94305, USA.

Blanchard Scott C

Kim Harold D

Puglisi Joseph D

Chu Steven

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.0705988104

Published

2007-08-21

Epub

2007-00-15

Pages

13661-5

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC1949337

Subset

Grants

NIGMS NIH HHS · R01 GM051266 · United States

NIGMS NIH HHS · GM51266 · United States

External Links

PubMed source DOI: 10.1073/pnas.0705988104 PMC Full Text View journal details

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