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PMID: 17709383 Published · ppublish English Journal Article

A highly polymorphic meiotic recombination mouse hot spot exhibits incomplete repair.

Molecular and cellular biology ·Vol. 27 ·No. 20 ·2007-10-00 ·Pages 7053-62

Bois PR

Abstract

The recent mapping of recombination hot spots in the human genome has demonstrated that crossover is a nonrandom process that occurs at well-defined positions along chromosomes. However, the mechanisms that direct hot-spot turnover in complex mammalian genomes are poorly understood. Analyses of the human genome are impaired by the inability to genetically dissect and molecularly manipulate recombinogenic regions to test their roles in regulating hot spots. Here, using the BXD recombinant inbred strains as a crossover library, three new recombination hot spots have been identified on mouse chromosome 19. Analyses of a highly polymorphic recombination hot spot (HS22) revealed that approximately 4% of recombinant molecules display complex and incomplete repair with discontinuous conversion tracts, as well as persistent heteroduplex DNA at crossover sites in mature spermatozoa. Also, sequence analysis of the wild house mouse revealed instability at the center of this hot spot. This suggests that complete repair is not required for completion of mammalian meiosis, a scenario that leaves duplex DNA containing mismatches at crossover sites.

MeSH Terms
Animals Base Sequence Chromosomes, Mammalian DNA/chemistry,genetics,metabolism DNA Repair Female Humans Male Meiosis/physiology Mice Mice, Inbred C57BL Microsatellite Repeats Molecular Sequence Data Nucleic Acid Conformation Polymorphism, Genetic Recombination, Genetic Sequence Alignment Spermatozoa/physiology
Chemicals
DNA
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Bois Philippe R J
Department of Cancer Biology, Genome Plasticity Laboratory, The Scripps Research Institute, 5353 Parkside Drive, RE-1, Jupiter, FL 33458, USA. [email protected]
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.00874-07
Email
[email protected]
Published
2007-10-00
Epub
2007-00-20
Pages
7053-62
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC2168899
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.00874-07 PMC Full Text View journal details
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