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PMID: 17709398 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

The Swi/Snf complex is important for histone eviction during transcriptional activation and RNA polymerase II elongation in vivo.

Molecular and cellular biology ·Vol. 27 ·No. 20 ·2007-10-00 ·Pages 6987-95

Schwabish MA, Struhl K

Abstract

The Swi/Snf nucleosome-remodeling complex is recruited by DNA-binding activator proteins, whereupon it alters chromatin structure to increase preinitiation complex formation and transcription. At the SUC2 promoter, the Swi/Snf complex is required for histone eviction in a manner that is independent of transcriptional activity. Swi/Snf travels through coding regions with elongating RNA polymerase (Pol) II, and swi2 mutants exhibit sensitivity to drugs affecting Pol elongation. In FACT-depleted cells, Swi/Snf is important for internal initiation within coding regions, suggesting that Swi/Snf is important for histone eviction that occurs during Pol II elongation. Taken together, these observations suggest that Swi/Snf is important for histone eviction at enhancers and that it also functions as a Pol II elongation factor.

MeSH Terms
Adenosine Triphosphatases DNA-Binding Proteins/genetics,metabolism Enhancer Elements, Genetic Histones/metabolism Humans Macromolecular Substances/metabolism Peptide Chain Elongation, Translational Peptide Elongation Factors/genetics,metabolism Promoter Regions, Genetic Protein Subunits/genetics,metabolism RNA Polymerase II/genetics,metabolism Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism Transcription Factors/genetics,metabolism Transcriptional Activation beta-Fructofuranosidase/genetics,metabolism
Chemicals
DNA-Binding Proteins Histones Macromolecular Substances Peptide Elongation Factors Protein Subunits Saccharomyces cerevisiae Proteins Transcription Factors RNA Polymerase II SUC2 protein, S cerevisiae beta-Fructofuranosidase Adenosine Triphosphatases SNF2 protein, S cerevisiae
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Schwabish Marc A
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA.
Struhl Kevin
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.00717-07
Published
2007-10-00
Epub
2007-00-20
Pages
6987-95
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC2168902
Subset
IM
Grants
NIGMS NIH HHS · R01 GM030186 · United States
NIGMS NIH HHS · R37 GM030186 · United States
NIGMS NIH HHS · GM30186 · United States
External Links
PubMed source DOI: 10.1128/MCB.00717-07 PMC Full Text View journal details
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