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PMID: 1901413 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Functional interdependence of the yeast SNF2, SNF5, and SNF6 proteins in transcriptional activation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 7 ·1991-04-01 ·Pages 2687-91

Laurent BC, Treitel MA, Carlson M

Abstract

The SNF2, SNF5, and SNF6 genes of Saccharomyces cerevisiae are required for expression of a variety of differently regulated genes. Previous evidence implicated the SNF5 protein in transcriptional activation, and a DNA-bound LexA-SNF5 fusion protein was shown to activate expression of a nearby promoter. Here, we examine the functional relationship of the SNF2, SNF5, and SNF6 proteins. Activation by DNA-bound LexA-SNF5 fusion protein was greatly reduced in snf2 and snf6 mutants, indicating that activation by LexA-SNF5 requires SNF2 and SNF6 function. An spt6 mutation, which suppresses transcriptional defects caused by snf2, restored activation by LexA-SNF5 in a snf2 mutant. The SNF2 gene was sequenced and encodes a 194-kDa protein that is targeted to the nucleus. DNA-bound LexA-SNF2 fusion protein also activated transcription, dependent on SNF5 and SNF6. These findings suggest that SNF2, SNF5, and SNF6 function interdependently in transcriptional activation, possibly forming a heteromeric complex.

MeSH Terms
Adenosine Triphosphatases Amino Acid Sequence Base Sequence Cell Nucleus/metabolism DNA, Fungal/genetics,isolation & purification DNA-Binding Proteins/genetics Gene Expression Regulation, Fungal Genes, Fungal Genotype Molecular Sequence Data Nuclear Proteins Plasmids Recombinant Fusion Proteins/metabolism Restriction Mapping Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Transcription Factors/genetics Transcription, Genetic beta-Galactosidase/genetics,metabolism
Chemicals
DNA, Fungal DNA-Binding Proteins Nuclear Proteins Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins Transcription Factors beta-Galactosidase Adenosine Triphosphatases SNF2 protein, S cerevisiae
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Laurent B C
Department of Genetics and Development, Columbia University College of Physicians and Surgeons, New York, NY 10032.
Treitel M A
Carlson M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.7.2687
Published
1991-04-01
Pages
2687-91
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC51303
Subset
IM
Grants
NIGMS NIH HHS · F32 GM11933 · United States
NIGMS NIH HHS · GM34095 · United States
Databases
GENBANK
M55906
External Links
PubMed source DOI: 10.1073/pnas.88.7.2687 PMC Full Text View journal details
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