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PMID: 2233708 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The SNF5 protein of Saccharomyces cerevisiae is a glutamine- and proline-rich transcriptional activator that affects expression of a broad spectrum of genes.

Molecular and cellular biology ·Vol. 10 ·No. 11 ·1990-11-00 ·Pages 5616-25

Laurent BC, Treitel MA, Carlson M

Abstract

The Saccharomyces cerevisiae SNF5 gene affects expression of both glucose- and phosphate-regulated genes and appears to function in transcription. We report the nucleotide sequence, which predicts that SNF5 encodes a 102,536-dalton protein. The N-terminal third of the protein is extremely rich in glutamine and proline. Mutants carrying a deletion of the coding sequence were viable but grew slowly, indicating that the SNF5 gene is important but not essential. Evidence that SNF5 affects expression of the cell type-specific genes MF alpha 1 and BAR1 at the RNA level extends the known range of SNF5 function. SNF5 is apparently required for expression of a wide variety of differently regulated genes. A bifunctional SNF5-beta-galactosidase fusion protein was localized in the nucleus by immunofluorescence. No DNA-binding activity was detected for SNF5. A LexA-SNF5 fusion protein, when bound to a lexA operator, functioned as a transcriptional activator.

Related Genes
SNF5
MeSH Terms
Amino Acid Sequence Base Sequence Cell Nucleus/metabolism Chromosomal Proteins, Non-Histone DNA-Binding Proteins/genetics,metabolism Gene Expression Regulation, Fungal Genes, Fungal Genotype Glutamine Molecular Sequence Data Plasmids Proline Restriction Mapping Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Transcription Factors/genetics,metabolism
Chemicals
Chromosomal Proteins, Non-Histone DNA-Binding Proteins SNF5 protein, S cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Glutamine Proline
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Laurent B C
Department of Genetics and Development, Columbia University College of Physicians and Surgeons, New York, New York 10032.
Treitel M A
Carlson M
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.11.5616-5625.1990
Published
1990-11-00
Pages
5616-25
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC361320
Subset
IM
Grants
NIGMS NIH HHS · F32 GM11933 · United States
NIGMS NIH HHS · GM34095 · United States
Databases
GENBANK
M36482
External Links
PubMed source DOI: 10.1128/mcb.10.11.5616-5625.1990 PMC Full Text View journal details
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