Home LiteratureArticle Details
PMID: 3900928 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Comparison of two yeast invertase genes: conservation of the upstream regulatory region.

Nucleic acids research ·Vol. 13 ·No. 17 ·1985-09-11 ·Pages 6089-103

Sarokin L, Carlson M

Abstract

The yeast genome contains a dispersed family of invertase structural genes (SUC1-SUC5, SUC7). Five of these genes are located very close to telomeres and are flanked by large regions of homologous sequence; recombination between telomeres could account for the dispersal of these SUC genes to different chromosomes. The SUC2 locus, in contrast, is not near a telomere and does not share large regions of flanking homology with the other loci. We examine here the relationship between SUC2 and one of the telomeric genes, SUC7. Sequence comparison revealed homology extending from about position -624 to +1791, which is close to the end of the mRNA. The 5' noncoding sequence includes two highly conserved regions: the region between -140 and +1, which contains the TATA box and presumably other promoter elements, and a second region extending from -508 to -400, which corresponds to the upstream regulatory region.

MeSH Terms
Amino Acid Sequence Base Sequence Genes Genes, Fungal Genes, Regulator Glycoside Hydrolases/genetics Nucleic Acid Hybridization Plasmids Saccharomyces cerevisiae/enzymology,genetics Sequence Homology, Nucleic Acid beta-Fructofuranosidase
Chemicals
Glycoside Hydrolases beta-Fructofuranosidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sarokin L
Carlson M
References (24)
24 references, click to expand
  1. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  2. Upstream region required for regulated expression of the glucose-repressible SUC2 gene of Saccharomyces cerevisiae.
    Mol Cell Biol. 1984 Dec;4(12):2750-7 PMID: 6396505
  3. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  4. SUC genes of yeast: a dispersed gene family.
    Cold Spring Harb Symp Quant Biol. 1981;45 Pt 2:799-803 PMID: 7021054
  5. Synthesis and processing of asparagine-linked oligosaccharides.
    Annu Rev Biochem. 1981;50:555-83 PMID: 7023366
  6. Presecretory and cytoplasmic invertase polypeptides encoded by distinct mRNAs derived from the same structural gene differ by a signal sequence.
    Proc Natl Acad Sci U S A. 1982 Feb;79(3):781-5 PMID: 7038684
  7. Two differentially regulated mRNAs with different 5' ends encode secreted with intracellular forms of yeast invertase.
    Cell. 1982 Jan;28(1):145-54 PMID: 7039847
  8. Mutants of yeast defective in sucrose utilization.
    Genetics. 1981 May;98(1):25-40 PMID: 7040163
  9. Genetic evidence for a silent SUC gene in yeast.
    Genetics. 1981 May;98(1):41-54 PMID: 7040164
  10. DNA sequence required for efficient transcription termination in yeast.
    Cell. 1982 Mar;28(3):563-73 PMID: 6280875
  11. Nucleotide sequence of the yeast SUC2 gene for invertase.
    Nucleic Acids Res. 1983 Mar 25;11(6):1943-54 PMID: 6300785
  12. Organization of the SUC gene family in Saccharomyces.
    Mol Cell Biol. 1983 Mar;3(3):351-9 PMID: 6843548
  13. The secreted form of invertase in Saccharomyces cerevisiae is synthesized from mRNA encoding a signal sequence.
    Mol Cell Biol. 1983 Mar;3(3):439-47 PMID: 6341817
  14. Establishing homologies in protein sequences.
    Methods Enzymol. 1983;91:524-45 PMID: 6855599
  15. Organization of DNA sequences and replication origins at yeast telomeres.
    Cell. 1983 Jun;33(2):563-73 PMID: 6345000
  16. Determination of enzymatic activity in polyacrylamide gels. I. Enzymes catalyzing the conversion of nonreducing substrates to reducing products.
    Anal Biochem. 1969 Mar;27(3):545-54 PMID: 4304181
  17. Beta-D-fructofuranoside fructohydrolase from yeast.
    Methods Enzymol. 1975;42:504-11 PMID: 237205
  18. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  19. Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5350-4 PMID: 414220
  20. Transcription terminates in yeast distal to a control sequence.
    Cell. 1983 Jun;33(2):607-14 PMID: 6305514
  21. Cloning and genetic mapping of SNF1, a gene required for expression of glucose-repressible genes in Saccharomyces cerevisiae.
    Mol Cell Biol. 1984 Jan;4(1):49-53 PMID: 6366512
  22. Role of an upstream regulatory element in leucine repression of the Saccharomyces cerevisiae leu2 gene.
    Nature. 1984 Feb 23-29;307(5953):740-2 PMID: 6321998
  23. Yeast LEU2. Repression of mRNA levels by leucine and primary structure of the gene product.
    J Biol Chem. 1984 Jul 10;259(13):8059-62 PMID: 6330094
  24. Faithful and efficient translation of homologous and heterologous mRNAs in an mRNA-dependent cell-free system from Saccharomyces cerevisiae.
    J Biol Chem. 1980 Sep 25;255(18):8761-6 PMID: 6997303
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.17.6089
Published
1985-09-11
Pages
6089-103
Language
English
Region
England
NLM ID
0411011
PMCID
PMC321940
Subset
IM
Grants
NIGMS NIH HHS · GM07088 · United States
NIGMS NIH HHS · GM32065 · United States
Databases
GENBANK
K00540, K03153, V01311
External Links
PubMed source DOI: 10.1093/nar/13.17.6089 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2