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PMID: 2201908 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

SPT6, an essential gene that affects transcription in Saccharomyces cerevisiae, encodes a nuclear protein with an extremely acidic amino terminus.

Molecular and cellular biology ·Vol. 10 ·No. 9 ·1990-09-00 ·Pages 4935-41

Swanson MS, Carlson M, Winston F

Abstract

SPT6 is an essential gene of Saccharomyces cerevisiae that appears to play a role in transcription. Mutations in the SPT6 (SSN20, CRE2) gene suppress delta insertion mutations in the 5' regions of HIS4 and LYS2 and mutations in cis- and/or trans-acting elements that are required for expression of SUC2 and ADH2. We report here that SPT6 encodes a 170-kilodalton highly charged protein with an extremely acidic amino terminus. By use of an epitope-tagged SPT6 protein, we have determined by indirect immunofluorescence that the SPT6 protein is located in the nucleus.

MeSH Terms
Amino Acid Sequence Base Sequence Fluorescent Antibody Technique Fungal Proteins/analysis,genetics Genes, Fungal Genotype Histone Chaperones Molecular Sequence Data Mutation Nuclear Proteins/genetics Restriction Mapping Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Suppression, Genetic Transcription, Genetic Transcriptional Elongation Factors
Chemicals
Fungal Proteins Histone Chaperones Nuclear Proteins SPT6 protein, S cerevisiae Saccharomyces cerevisiae Proteins Transcriptional Elongation Factors
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Swanson M S
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115.
Carlson M
Winston F
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.9.4935-4941.1990
Published
1990-09-00
Pages
4935-41
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC361114
Subset
IM
Grants
NIGMS NIH HHS · GM32967 · United States
NIGMS NIH HHS · GM34095 · United States
NIGMS NIH HHS · T32-GM07088 · United States
Databases
GENBANK
M34391
External Links
PubMed source DOI: 10.1128/mcb.10.9.4935-4941.1990 PMC Full Text View journal details
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