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PMID: 1782677 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Two potential Petunia hybrida mitochondrial DNA replication origins show structural and in vitro functional homology with the animal mitochondrial DNA heavy and light strand replication origins.

Current genetics ·Vol. 20 ·No. 6 ·1991-12-00 ·Pages 503-13

de Haas JM, Hille J, Kors F, van der Meer B, Kool AJ, Folkerts O, Nijkamp HJ

Abstract

Four Petunia hybrida mitochondrial (mt) DNA fragments have been isolated, sequenced, localized on the physical map and analyzed for their ability to initiate specific DNA synthesis. When all four mtDNA fragments were tested as templates in an in vitro DNA synthesizing lysate system, developed from purified P. hybrida mitochondria, specific initiation of DNA synthesis could only be observed starting within two fragments, oriA and oriB. When DNA synthesis incubations were performed with DNA templates consisting of both the A and B origins in the same plasmid in complementary strands, DNA synthesis first initiates in the A-origin, proceeds in the direction of the B-origin after which replication is also initiated in the B-origin. Based on these observations, a replication model for the P. hybrida mitochondrial genome is presented.

MeSH Terms
Animals Base Sequence Cloning, Molecular DNA/ultrastructure DNA Replication DNA, Mitochondrial/genetics Humans Microscopy, Electron Molecular Sequence Data Organelles Plants Plasmids Restriction Mapping Sequence Homology, Nucleic Acid
Chemicals
DNA, Mitochondrial DNA
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
de Haas J M
Vrije Universiteit, Department of Genetics, Amsterdam, The Netherlands.
Hille J
Kors F
van der Meer B
Kool A J
Folkerts O
Nijkamp H J
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Article Info
Journal
Current genetics
Abbr.
Curr Genet
ISSN
0172-8083
DOI
10.1007/BF00334779
Published
1991-12-00
Pages
503-13
Language
English
Region
United States
NLM ID
8004904
Subset
IM
Databases
GENBANK
S39777, S60919, S60920, S81533, S81534, S81536, X15105, X15106, X15107, X59717, X59718, X62800
External Links
PubMed source DOI: 10.1007/BF00334779 View journal details
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