Generation of superoxide anion and chemiluminescence by human monocytes during phagocytosis and on contact with surface-bound immunoglobulin G.

The Journal of experimental medicine ·Vol. 143 ·No. 6 ·1976-06-01 ·Pages 1551-6

Johnston RB, Lehmeyer JE, Guthrie LA

Abstract

Extent of O-2-release and chemiluminescence, attributed to singlet oxygen, has been compared in human monocytes and neutrophils during phagocytosis, stimulation by the surface-active agent phorbol myristate acetate, or contact with aggregated IgG in a model of immune complex disease. Monocytes generated O-2-and chemiluminescence with each of the three stimuli, although values were significantly less than those of neutrophils from the same individuals. Lymphocytes had no significant activity in either assay with any stimulus. Oxygen metabolites released from mononuclear phagocytes are highly reactive and could play a part in both the beneficial and detrimental aspects of chronic inflammation.

MeSH Terms

Immunoglobulin G Luminescent Measurements Monocytes/immunology,metabolism Oxygen/biosynthesis Phagocytosis Receptors, Antigen, B-Cell Superoxides/biosynthesis Tetradecanoylphorbol Acetate/pharmacology Zymosan

Chemicals

Immunoglobulin G Receptors, Antigen, B-Cell Superoxides Zymosan Tetradecanoylphorbol Acetate Oxygen

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Johnston R B

Lehmeyer J E

Guthrie L A

References (13)

13 references, click to expand

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Article Info

Journal

The Journal of experimental medicine

Abbr.

J Exp Med

ISSN

0022-1007

DOI

10.1084/jem.143.6.1551

Published

1976-06-01

Pages

1551-6

Language

English

Region

United States

NLM ID

2985109R

PMCID

PMC2190206

Subset

External Links

PubMed source DOI: 10.1084/jem.143.6.1551 PMC Full Text View journal details

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Generation of superoxide anion and chemiluminescence by human monocytes during phagocytosis and on contact with surface-bound immunoglobulin G.

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