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PMID: 181401 Published · ppublish English Journal Article

Elaboration of toxic oxygen by-products by neutrophils in a model of immune complex disease.

The Journal of clinical investigation ·Vol. 57 ·No. 4 ·1976-04-00 ·Pages 836-41

Johnston RB, Lehmeyer JE

Abstract

Contact between human neutrophils and aggregated immunoglobulin G bound to micropore filters has been studied as a model of the pathogenesis of tissue damage in immune complex disease. Contact with this surface, as well as with plain filters and polystyrene petri dishes, induced neutrophils to elaborate superoxide anion and hydrogen peroxide and to generate chemiluminescence, which has been attributed to singlet oxygen. Pretreatment of the cells with cytochalasin B decreased these activities but increased release of lysosomal beta-glucuronidase, suggesting that degranulation and the burst of oxygen metabolism that characterizes phagocytes are independently regulated functions. Toxic oxygen metabolites released from neutrophils are highly reactive and could mediate tissue injury at sites of inflammation.

MeSH Terms
Anions Cell Nucleus Cytochalasin B/pharmacology Hexosephosphates/blood Humans Hydrogen Peroxide/metabolism Immune Complex Diseases Immunoglobulin G/metabolism Immunoglobulins/metabolism Luminescent Measurements Micropore Filters Models, Biological Neutrophils/enzymology Oxygen/metabolism Pentosephosphates/blood Phagocytosis Polystyrenes Superoxides/metabolism
Chemicals
Anions Hexosephosphates Immunoglobulin G Immunoglobulins Pentosephosphates Polystyrenes Superoxides Cytochalasin B Hydrogen Peroxide Oxygen
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Johnston R B
Lehmeyer J E
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38 references, click to expand
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI108359
Published
1976-04-00
Pages
836-41
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC436726
Subset
IM
External Links
PubMed source DOI: 10.1172/JCI108359 PMC Full Text View journal details
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