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PMID: 17898064 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

HC-Pro protein of Potato virus Y can interact with three Arabidopsis 20S proteasome subunits in planta.

Journal of virology ·Vol. 81 ·No. 23 ·2007-12-00 ·Pages 12881-8

Jin Y, Ma D, Dong J, Jin J, Li D, Deng C, Wang T

Abstract

The multifunctional protein helper component proteinase (HC-Pro) is thought to interfere with the activity of the 20S proteasome; however, no sites of interaction have been identified for either protein. Here, we first show that the Potato virus Y (PVY) HC-Pro protein can interact with three Arabidopsis 20S proteasome subunits (PAA, PBB, and PBE), using a yeast two-hybrid system and the bimolecular fluorescence complement assay. In addition, yeast two-hybrid analysis of the interaction between several mutant subunits of the 20S proteasome and PVY HC-Pro confirmed that residues 81 to 140 of PAA, 1 to 80 of PBB, and 160 to 274 of PBE are necessary for binding PAA, PBB, and PBE to PVY HC-Pro, respectively. Deletion mutant analysis of PVY HC-Pro showed that the N terminus (residues 1 to 97) is necessary for its interaction with three Arabidopsis 20S proteasome subunits. The ability of HC-Pro to interact and interfere with the activity of the 20S proteasome may help explain the molecular basis of its multifunctional character.

MeSH Terms
Amino Acid Substitution/genetics Arabidopsis/virology Cysteine Endopeptidases/metabolism Mutation, Missense Plant Proteins/metabolism Potyvirus/physiology Proteasome Endopeptidase Complex/genetics,metabolism Protein Binding Protein Interaction Mapping Sequence Deletion Two-Hybrid System Techniques Viral Proteins/metabolism
Chemicals
Plant Proteins Viral Proteins Cysteine Endopeptidases HC-Pro protein, potyvirus Proteasome Endopeptidase Complex
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Jin Yongsheng
State Key Laboratory of Agro-biotechnology, China Agricultural University, 2 Yuanmingyuan West Road, Haidian District, Beijing 100094, PR China.
Ma Dongyuan
Dong Jiangli
Jin Jingchen
Li Daofeng
Deng Changwang
Wang Tao
References (39)
39 references, click to expand
  1. Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis.
    Nat Biotechnol. 2003 May;21(5):539-45 PMID: 12692560
  2. Proteasome complex as a potential cellular target of hepatitis B virus X protein.
    J Virol. 1996 Aug;70(8):5582-91 PMID: 8764072
  3. The ubiquitin/26S proteasome pathway, the complex last chapter in the life of many plant proteins.
    Trends Plant Sci. 2003 Mar;8(3):135-42 PMID: 12663224
  4. Repeat sequence of Epstein-Barr virus-encoded nuclear antigen 1 protein interrupts proteasome substrate processing.
    J Biol Chem. 2004 Mar 5;279(10):8635-41 PMID: 14688254
  5. Stability in vitro of the 69K movement protein of Turnip yellow mosaic virus is regulated by the ubiquitin-mediated proteasome pathway.
    J Gen Virol. 2002 Dec;83(Pt 12):3187-97 PMID: 12466497
  6. Evidence for phosphorylation and ubiquitinylation of the turnip yellow mosaic virus RNA-dependent RNA polymerase domain expressed in a baculovirus-insect cell system.
    Biochem J. 2000 Jul 15;349(Pt 2):417-25 PMID: 10880340
  7. The diverse roles of ubiquitin and the 26S proteasome in the life of plants.
    Nat Rev Genet. 2003 Dec;4(12):948-58 PMID: 14631355
  8. Structure and functions of the 20S and 26S proteasomes.
    Annu Rev Biochem. 1996;65:801-47 PMID: 8811196
  9. An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of tomato bushy stunt virus.
    Plant J. 2003 Mar;33(5):949-56 PMID: 12609035
  10. HcPro, a multifunctional protein encoded by a plant RNA virus, targets the 20S proteasome and affects its enzymic activities.
    J Gen Virol. 2005 Sep;86(Pt 9):2595-603 PMID: 16099919
  11. HIV-1 tat inhibits the 20 S proteasome and its 11 S regulator-mediated activation.
    J Biol Chem. 1997 Mar 28;272(13):8145-8 PMID: 9079628
  12. Substrate affinity and substrate specificity of proteasomes with RNase activity.
    Mol Biol Rep. 2003 Mar;30(1):1-7 PMID: 12688529
  13. Rapid one-step protein purification from plant material using the eight-amino acid StrepII epitope.
    Plant Mol Biol. 2004 May;55(1):135-47 PMID: 15604670
  14. Plant development: regulation by protein degradation.
    Science. 2002 Aug 2;297(5582):793-7 PMID: 12161644
  15. Hepatitis B virus X protein is both a substrate and a potential inhibitor of the proteasome complex.
    J Virol. 1999 Sep;73(9):7231-40 PMID: 10438810
  16. Misfolded plant virus proteins: elicitors and targets of ubiquitylation.
    FEBS Lett. 2003 Jun 19;545(2-3):229-32 PMID: 12804781
  17. Visualization of protein interactions in living plant cells using bimolecular fluorescence complementation.
    Plant J. 2004 Nov;40(3):428-38 PMID: 15469500
  18. Biochemical identification of proteasome-associated endonuclease activity in sunflower.
    Biochim Biophys Acta. 2003 Jan 31;1645(1):30-9 PMID: 12535608
  19. A viral suppressor of gene silencing in plants.
    Proc Natl Acad Sci U S A. 1998 Oct 27;95(22):13079-84 PMID: 9789044
  20. Plant viral suppressors of RNA silencing.
    Virus Res. 2004 Jun 1;102(1):97-108 PMID: 15068885
  21. Ubiquitin, proteasomes, and the regulation of intracellular protein degradation.
    Curr Opin Cell Biol. 1995 Apr;7(2):215-23 PMID: 7612274
  22. The ubiquitin-proteasome pathway and plant development.
    Plant Cell. 2004 Dec;16(12):3181-95 PMID: 15579807
  23. P1/HC-Pro, a viral suppressor of RNA silencing, interferes with Arabidopsis development and miRNA unction.
    Dev Cell. 2003 Feb;4(2):205-17 PMID: 12586064
  24. Human immunodeficiency virus-1 Tat protein interacts with distinct proteasomal alpha and beta subunits.
    FEBS Lett. 2003 Oct 9;553(1-2):200-4 PMID: 14550573
  25. Degradation of the epstein-barr virus latent membrane protein 1 (LMP1) by the ubiquitin-proteasome pathway. Targeting via ubiquitination of the N-terminal residue.
    J Biol Chem. 2000 Aug 4;275(31):23491-9 PMID: 10807912
  26. The ubiquitin 26S proteasome proteolytic pathway.
    Annu Rev Plant Biol. 2004;55:555-90 PMID: 15377232
  27. Molecular organization of the 20S proteasome gene family from Arabidopsis thaliana.
    Genetics. 1998 Jun;149(2):677-92 PMID: 9611183
  28. The RTP site shared by the HIV-1 Tat protein and the 11S regulator subunit alpha is crucial for their effects on proteasome function including antigen processing.
    J Mol Biol. 2002 Nov 1;323(4):771-82 PMID: 12419264
  29. Structural and functional characterization of interaction between hepatitis B virus X protein and the proteasome complex.
    J Biol Chem. 2000 May 19;275(20):15157-65 PMID: 10748218
  30. Physical and functional interactions between pathogen-induced Arabidopsis WRKY18, WRKY40, and WRKY60 transcription factors.
    Plant Cell. 2006 May;18(5):1310-26 PMID: 16603654
  31. Potyvirus proteins: a wealth of functions.
    Virus Res. 2001 Apr;74(1-2):157-75 PMID: 11226583
  32. Proteasomes (prosomes) inhibit the translation of tobacco mosaic virus RNA by preventing the formation of initiation complexes.
    Mol Biol Rep. 1994;20(2):57-61 PMID: 7715610
  33. Mechanisms underlying ubiquitination.
    Annu Rev Biochem. 2001;70:503-33 PMID: 11395416
  34. Ectopic DICER-LIKE1 expression in P1/HC-Pro Arabidopsis rescues phenotypic anomalies but not defects in microRNA and silencing pathways.
    Plant Cell. 2005 Nov;17(11):2873-85 PMID: 16214897
  35. Proteolysis in plants: mechanisms and functions.
    Plant Mol Biol. 1996 Oct;32(1-2):275-302 PMID: 8980483
  36. Viral pathogenicity determinants are suppressors of transgene silencing in Nicotiana benthamiana.
    EMBO J. 1998 Nov 16;17(22):6739-46 PMID: 9822616
  37. Substrate access and processing by the 20S proteasome core particle.
    Int J Biochem Cell Biol. 2003 May;35(5):606-16 PMID: 12672453
  38. Visualization of interactions among bZIP and Rel family proteins in living cells using bimolecular fluorescence complementation.
    Mol Cell. 2002 Apr;9(4):789-98 PMID: 11983170
  39. Structural characterization of HC-Pro, a plant virus multifunctional protein.
    J Biol Chem. 2003 Jun 27;278(26):23753-61 PMID: 12684502
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
1098-5514
DOI
10.1128/JVI.00913-07
Published
2007-12-00
Epub
2007-00-26
Pages
12881-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC2169110
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.00913-07 PMC Full Text View journal details
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