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PMID: 8764072 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Proteasome complex as a potential cellular target of hepatitis B virus X protein.

Journal of virology ·Vol. 70 ·No. 8 ·1996-08-00 ·Pages 5582-91

Huang J, Kwong J, Sun EC, Liang TJ

Abstract

Although the biological importance of hepatitis B virus X protein (HBX) in the life cycle of hepatitis B virus has been well established, the cellular and molecular basis of its function remains largely undefined. Despite the association of multiple activities with HBX, none of them appear to provide a unifying hypothesis regarding the true biological function of HBX. Identification and characterization of cellular targets of HBX remain an essential goal in the elucidation of the molecular mechanisms of HBX. Using the Saccharomyces cerevisiae two-hybrid system, we have identified and characterized a novel subunit of the proteasome complex (XAPC7) that interacts specifically with HBX. We also showed that HBX binds specifically to XAPC7 in vitro. Mutagenesis studies have defined the domains of interaction to be critical for the function of HBX. Furthermore, overexpression of XAPC7 appeared to activate transcription by itself and antisense expression of XAPC7 was able to block transactivation by HBX. Therefore, the proteasome complex is possibly a functional target of HBX in cells.

MeSH Terms
Amino Acid Sequence Base Sequence Binding Sites Cysteine Endopeptidases/genetics,metabolism DNA, Complementary Hepatitis B virus/metabolism Molecular Sequence Data Multienzyme Complexes/genetics,metabolism Mutagenesis, Site-Directed Peptide Mapping Proteasome Endopeptidase Complex Saccharomyces cerevisiae Trans-Activators/metabolism Viral Regulatory and Accessory Proteins
Chemicals
DNA, Complementary Multienzyme Complexes Trans-Activators Viral Regulatory and Accessory Proteins hepatitis B virus X protein Cysteine Endopeptidases Proteasome Endopeptidase Complex
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Huang J
Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, 02114, USA.
Kwong J
Sun E C
Liang T J
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.70.8.5582-5591.1996
Published
1996-08-00
Pages
5582-91
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC190518
Subset
IM
Grants
NCI NIH HHS · CA54524 · United States
NIDDK NIH HHS · DK01952 · United States
NIDDK NIH HHS · P30DK-43351 · United States
Databases
GENBANK
AF022815
External Links
PubMed source DOI: 10.1128/JVI.70.8.5582-5591.1996 PMC Full Text View journal details
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