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PMID: 17936713 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Yeast Rad5 protein required for postreplication repair has a DNA helicase activity specific for replication fork regression.

Molecular cell ·Vol. 28 ·No. 1 ·2007-10-12 ·Pages 167-75

Blastyák A, Pintér L, Unk I, Prakash L, Prakash S, Haracska L

Abstract

Lesions in the template DNA strand block the progression of the replication fork. In the yeast Saccharomyces cerevisiae, replication through DNA lesions is mediated by different Rad6-Rad18-dependent means, which include translesion synthesis and a Rad5-dependent postreplicational repair pathway that repairs the discontinuities that form in the DNA synthesized from damaged templates. Although translesion synthesis is well characterized, little is known about the mechanisms that modulate Rad5-dependent postreplicational repair. Here we show that yeast Rad5 has a DNA helicase activity that is specialized for replication fork regression. On model replication fork structures, Rad5 concertedly unwinds and anneals the nascent and the parental strands without exposing extended single-stranded regions. These observations provide insight into the mechanism of postreplicational repair in which Rad5 action promotes template switching for error-free damage bypass.

MeSH Terms
Adenosine Triphosphatases/genetics,metabolism DNA Helicases/genetics,metabolism DNA Repair DNA Replication DNA, Fungal/chemistry,genetics,metabolism Nucleic Acid Conformation Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism
Chemicals
DNA, Fungal Saccharomyces cerevisiae Proteins Adenosine Triphosphatases RAD5 protein, S cerevisiae DNA Helicases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Blastyák András
Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, Szeged, Temesvari krt.62, H-6726, Hungary.
Pintér Lajos
Unk Ildiko
Prakash Louise
Prakash Satya
Haracska Lajos
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Article Info
Journal
Molecular cell
Abbr.
Mol Cell
ISSN
1097-2765
DOI
10.1016/j.molcel.2007.07.030
Published
2007-10-12
Pages
167-75
Language
English
Region
United States
NLM ID
9802571
PMCID
PMC2034406
Subset
IM
Grants
NCI NIH HHS · R01 CA107650 · United States
Wellcome Trust · United Kingdom
NCI NIH HHS · CA 107650 · United States
Corrections
CommentIn
PMID: 17964257
External Links
PubMed source DOI: 10.1016/j.molcel.2007.07.030 PMC Full Text View journal details
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