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PMID: 17940093 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Trypanosome MTR4 is involved in rRNA processing.

Nucleic acids research ·Vol. 35 ·No. 20 ·2007-00-00 ·Pages 7023-30

Cristodero M, Clayton CE

Abstract

The yeast putative RNA helicase Mtr4p is implicated in exosome-mediated RNA quality control in the nucleus, interacts with the exosome, and is found in the 'TRAMP' complex with a yeast nuclear poly(A) polymerase (Trf4p/Pap2p or Trf5p) and a putative RNA-binding protein, Air1p or Air2p. Depletion of the Trypanosoma brucei MTR4-like protein TbMTR4 caused growth arrest and defects in 5.8S rRNA processing similar to those seen after depletion of the exosome. TbNPAPL, a nuclear protein which is a putative homolog of Trf4p/Pap2p, was required for normal cell growth. Depletion of MTR4 resulted in the accumulation of polyadenylated rRNA precursors, while depletion of TbNPAPL had little effect. These results suggest that polyadenylation-dependent nuclear rRNA quality control is conserved in eukaryotic evolution. In contrast, there was no evidence for a trypanosome TRAMP complex since no stable interactions between TbMTR4 and the exosome, TbNPAPL or RNA-binding proteins were detected.

MeSH Terms
Animals RNA Helicases/metabolism RNA Processing, Post-Transcriptional RNA, Ribosomal/metabolism Trypanosoma brucei brucei/genetics,metabolism
Chemicals
RNA, Ribosomal RNA Helicases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Cristodero Marina
Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), Im Neuenheimer Feld 282, 69120 Heidelberg, Germany.
Clayton Christine E
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/gkm736
Published
2007-00-00
Epub
2007-00-16
Pages
7023-30
Language
English
Region
England
NLM ID
0411011
PMCID
PMC2175323
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/gkm736 PMC Full Text View journal details
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