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PMID: 16896205 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization and developmentally regulated localization of the mitochondrial carrier protein homologue MCP6 from Trypanosoma brucei.

Eukaryotic cell ·Vol. 5 ·No. 8 ·2006-08-00 ·Pages 1194-205

Colasante C, Alibu VP, Kirchberger S, Tjaden J, Clayton C, Voncken F

Abstract

Proteins of the mitochondrial carrier family (MCF) are located mainly in the inner mitochondrial membrane and mediate the transport of a large range of metabolic intermediates. The genome of Trypanosoma brucei harbors 29 genes encoding different MCF proteins. We describe here the characterization of MCP6, a novel T. brucei MCF protein. Sequence comparison and phylogenetic reconstruction revealed that MCP6 is closely related to different mitochondrial ADP/ATP and calcium-dependent solute carriers, including the ATP-Mg/Pi carrier of Homo sapiens. However, MCP6 lacks essential amino acids and sequence motifs conserved in these metabolite transporters, and functional reconstitution and transport assays with E. coli suggested that this protein indeed does not function as an ADP/ATP or ATP-Mg/Pi carrier. The subcellular localization of MCP6 is developmentally regulated: in bloodstream-form trypanosomes, the protein is predominantly glycosomal, whereas in the procyclic form, it is found mainly in the mitochondria. Depletion of MCP6 in procyclic trypanosomes resulted in growth inhibition, an increased cell size, aberrant numbers of nuclei and kinetoplasts, and abnormal kinetoplast morphology, suggesting that depletion of MCP6 inhibits division of the kinetoplast.

MeSH Terms
Animals Base Sequence Escherichia coli/genetics Gene Expression Regulation, Developmental Humans Mitochondrial Membranes/chemistry Mitochondrial Proteins/chemistry,genetics Molecular Sequence Data Phylogeny Protozoan Proteins/chemistry,genetics RNA, Messenger/biosynthesis Sequence Alignment Sequence Homology, Amino Acid Trypanosoma brucei brucei/genetics,growth & development
Chemicals
Mitochondrial Proteins Protozoan Proteins RNA, Messenger
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Colasante Claudia
Zentrum für Molekulare Biologie (ZMBH), Im Neuenheimer Feld 282, D-69120 Heidelberg, Germany.
Alibu Vincent P
Kirchberger Simon
Tjaden Joachim
Clayton Christine
Voncken Frank
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Article Info
Journal
Eukaryotic cell
Abbr.
Eukaryot Cell
ISSN
1535-9778
DOI
10.1128/EC.00096-06
Published
2006-08-00
Pages
1194-205
Language
English
Region
United States
NLM ID
101130731
PMCID
PMC1539146
Subset
IM
External Links
PubMed source DOI: 10.1128/EC.00096-06 PMC Full Text View journal details
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