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PMID: 18197411 Published · ppublish English Journal Article Review

Global regulation of genome duplication in eukaryotes: an overview from the epifluorescence microscope.

Chromosoma ·Vol. 117 ·No. 3 ·2008-06-00 ·Pages 243-60

Herrick J, Bensimon A

Abstract

In eukaryotes, DNA replication is initiated along each chromosome at multiple sites called replication origins. Locally, each replication origin is "licensed" or specified at the end of the M and the beginning of the G1 phases of the cell cycle. During the S phase when DNA synthesis takes place, origins are activated in stages corresponding to early and late-replicating domains. The staged and progressive activation of replication origins reflects the need to maintain a strict balance between the number of active replication forks and the rate at which DNA synthesis proceeds. This suggests that origin densities (frequency of initiation) and replication fork movement (rates of elongation) must be coregulated to guarantee the efficient and complete duplication of each subchromosomal domain. Emerging evidence supports this proposal and suggests that the ATM/ATR intra-S phase checkpoint plays an important role in the coregulation of initiation frequencies and rates of elongation. In this paper, we review recent results concerning the mechanisms governing the global regulation of DNA replication and discuss the roles these mechanisms play in maintaining genome stability during both a normal and perturbed S phase.

MeSH Terms
Animals DNA Replication Eukaryotic Cells/metabolism Gene Duplication Genome/genetics Microscopy/methods Replicon
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Herrick John
Genomic Vision, 29, rue Faubourg St. Jacques, Paris 75014, France. [email protected]
Bensimon Aaron
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Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/s00412-007-0145-1
Email
[email protected]
Published
2008-06-00
Epub
2008-00-16
Pages
243-60
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
External Links
PubMed source DOI: 10.1007/s00412-007-0145-1 View journal details
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