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PMID: 1826351 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

E1A dependent up-regulation of c-jun/AP-1 activity.

Nucleic acids research ·Vol. 19 ·No. 3 ·1991-02-11 ·Pages 649-55

Kitabayashi I, Chiu R, Gachelin G, Yokoyama K

Abstract

E1A, the early region 1A transcription unit of human adenovirus, exhibits multiple functions that regulate the expression of some cellular genes and promote cell growth and division. We found that E1A stimulated c-jun gene expression at least fifty-fold in rat 3Y1 cells in a serum-independent manner, concomitantly with E1A down-regulation of jun B expression. The E1A-dependent induction of c-jun transcription resulted in increase amount of cJun/AP1. This induction was mediated by the enhancement of the binding activity of the transcription factor cJun/AP1 to an AP1 binding site in the c-jun promoter. Additionally, this induction can be repressed by introducing junB into the cells. Taken collectively, these results suggest that the differential expression of two closely related proteins greatly expands their cellular regulation. Induction of c-jun expression by E1A as well as c-jun autoregulation may amplify the action of E1A during adenovirus infection. Therefore, some of the biological effects of E1A may include mediating the constitutive activation of c-jun, which is important in transcriptional regulation and oncogenic transformation.

Related Genes
c-jun junB
MeSH Terms
Adenovirus Early Proteins Animals Blotting, Northern Cells, Cultured Cloning, Molecular DNA/metabolism DNA-Binding Proteins/immunology,physiology Gene Expression Regulation Oncogene Proteins, Viral/physiology Precipitin Tests Promoter Regions, Genetic Protein Binding Proto-Oncogene Proteins/immunology,physiology Proto-Oncogene Proteins c-jun Rats Regulatory Sequences, Nucleic Acid Transcription Factors/immunology,physiology Transcription, Genetic
Chemicals
Adenovirus Early Proteins DNA-Binding Proteins Oncogene Proteins, Viral Proto-Oncogene Proteins Proto-Oncogene Proteins c-jun Transcription Factors DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kitabayashi I
Tsukuba Life Science Center, Institute of Physical and Chemical Research (RIKEN), Ibaraki, Japan.
Chiu R
Gachelin G
Yokoyama K
References (52)
52 references, click to expand
  1. Human proto-oncogene c-jun encodes a DNA binding protein with structural and functional properties of transcription factor AP-1.
    Science. 1987 Dec 4;238(4832):1386-92 PMID: 2825349
  2. Identification of a cyclic-AMP-responsive element within the rat somatostatin gene.
    Proc Natl Acad Sci U S A. 1986 Sep;83(18):6682-6 PMID: 2875459
  3. Adenovirus promoters and E1A transactivation.
    Annu Rev Genet. 1986;20:45-79 PMID: 3028247
  4. Purified transcription factor AP-1 interacts with TPA-inducible enhancer elements.
    Cell. 1987 Jun 19;49(6):741-52 PMID: 3034433
  5. Activation of transcription factor IIIC by the adenovirus E1A protein.
    Cell. 1988 Jun 17;53(6):907-20 PMID: 2968157
  6. An adenovirus E1a protein region required for transformation and transcriptional repression.
    Cell. 1986 Sep 26;46(7):1043-51 PMID: 2944600
  7. E1A 13S and 12S mRNA products made in Escherichia coli both function as nucleus-localized transcription activators but do not directly bind DNA.
    Mol Cell Biol. 1985 Oct;5(10):2653-61 PMID: 2942760
  8. Promoter trans-activation of protooncogenes c-fos and c-myc, but not c-Ha-ras, by products of adenovirus early region 1A.
    Proc Natl Acad Sci U S A. 1987 Sep;84(18):6430-3 PMID: 2957694
  9. Fos-associated protein p39 is the product of the jun proto-oncogene.
    Science. 1988 May 20;240(4855):1010-6 PMID: 3130660
  10. The c-Fos protein interacts with c-Jun/AP-1 to stimulate transcription of AP-1 responsive genes.
    Cell. 1988 Aug 12;54(4):541-52 PMID: 3135940
  11. DNA binding activities of three murine Jun proteins: stimulation by Fos.
    Cell. 1988 Dec 2;55(5):907-15 PMID: 3142691
  12. The jun proto-oncogene is positively autoregulated by its product, Jun/AP-1.
    Cell. 1988 Dec 2;55(5):875-85 PMID: 3142689
  13. Induction of proto-oncogene JUN/AP-1 by serum and TPA.
    Nature. 1988 Aug 18;334(6183):629-31 PMID: 2457172
  14. Induction of transcription factor AP-1 by adenovirus E1A protein and cAMP.
    Genes Dev. 1989 Dec;3(12A):1991-2002 PMID: 2559873
  15. DNA-binding activity of the adenovirus-induced E4F transcription factor is regulated by phosphorylation.
    Genes Dev. 1989 May;3(5):620-7 PMID: 2545525
  16. Leucine zipper structure of the protein CRE-BP1 binding to the cyclic AMP response element in brain.
    EMBO J. 1989 Jul;8(7):2023-8 PMID: 2529117
  17. Adenovirus E1A protein paradigm viral transactivator.
    Annu Rev Genet. 1989;23:141-61 PMID: 2533472
  18. jun-B inhibits and c-fos stimulates the transforming and trans-activating activities of c-jun.
    Cell. 1989 Dec 22;59(6):987-97 PMID: 2513129
  19. Jun-B differs in its biological properties from, and is a negative regulator of, c-Jun.
    Cell. 1989 Dec 22;59(6):979-86 PMID: 2513128
  20. Characterization of junD: a new member of the jun proto-oncogene family.
    EMBO J. 1989 May;8(5):1433-9 PMID: 2504580
  21. jun-D: a third member of the jun gene family.
    Proc Natl Acad Sci U S A. 1989 Mar;86(5):1500-3 PMID: 2493644
  22. G1 events and regulation of cell proliferation.
    Science. 1989 Nov 3;246(4930):603-8 PMID: 2683075
  23. Deregulated expression of human c-jun transforms primary rat embryo cells in cooperation with an activated c-Ha-ras gene and transforms rat-1a cells as a single gene.
    Proc Natl Acad Sci U S A. 1989 Apr;86(7):2257-61 PMID: 2648396
  24. Structure and chromosomal localization of the functional intronless human JUN protooncogene.
    Proc Natl Acad Sci U S A. 1988 Dec;85(23):9148-52 PMID: 3194415
  25. Oncogene jun encodes a sequence-specific trans-activator similar to AP-1.
    Nature. 1988 Mar 10;332(6160):166-71 PMID: 3347253
  26. HeLa cell beta-tubulin gene transcription is stimulated by adenovirus 5 in parallel with viral early genes by an E1a-dependent mechanism.
    Mol Cell Biol. 1984 Dec;4(12):2792-801 PMID: 6527689
  27. Monoclonal antibodies specific for adenovirus early region 1A proteins: extensive heterogeneity in early region 1A products.
    J Virol. 1985 Sep;55(3):533-46 PMID: 3894685
  28. Identification of sequences responsible for positive and negative regulation by E1A in the promoter of H-2Kbm1 class I MHC gene.
    EMBO J. 1990 Jan;9(1):127-35 PMID: 2136829
  29. Activation of an intron enhancer within the keratin 18 gene by expression of c-fos and c-jun in undifferentiated F9 embryonal carcinoma cells.
    Genes Dev. 1990 May;4(5):835-48 PMID: 1696235
  30. General repression of enhanson activity by the adenovirus-2 E1A proteins.
    Genes Dev. 1990 Jan;4(1):137-50 PMID: 2155159
  31. A specific member of the ATF transcription factor family can mediate transcription activation by the adenovirus E1a protein.
    Cell. 1990 Jun 29;61(7):1217-24 PMID: 2142019
  32. Direct cloning of leucine zipper proteins: Jun binds cooperatively to the CRE with CRE-BP1.
    Oncogene. 1990 Apr;5(4):451-8 PMID: 2139203
  33. Nucleotide sequence of rat c-jun protooncogene.
    Nucleic Acids Res. 1990 Jun 11;18(11):3400 PMID: 2113275
  34. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  35. Expression of the E4 gene is required for establishment of soft-agar colony-forming rat cell lines transformed by the adenovirus 12 E1 gene.
    J Virol. 1984 Jun;50(3):854-63 PMID: 6328016
  36. Structure and gene organization in the transformed Hind III-G fragment of Ad12.
    Cell. 1980 Jul;20(3):777-86 PMID: 6251973
  37. Analysis of adenovirus transforming proteins from early regions 1A and 1B with antisera to inducible fusion antigens produced in Escherichia coli.
    J Virol. 1984 Jan;49(1):132-41 PMID: 6361277
  38. An adenovirus type 5 early gene function regulates expression of other early viral genes.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3665-9 PMID: 291030
  39. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
  40. Pre-early adenovirus 5 gene product regulates synthesis of early viral messenger RNAs.
    Cell. 1979 Aug;17(4):935-44 PMID: 487437
  41. Size and location of the transforming region in human adenovirus type 5 DNA.
    Nature. 1974 Oct 25;251(5477):687-91 PMID: 4473721
  42. Rat cell line 3y1 and its virogenic polyoma- and sv40- transformed derivatives.
    Int J Cancer. 1975 Apr 15;15(4):694-706 PMID: 166944
  43. Elevation of AP1 activity during F9 cell differentiation is due to increased c-jun transcription.
    New Biol. 1990 Apr;2(4):351-61 PMID: 1963081
  44. Differential effects of the adenovirus E1A oncogene on members of the AP-1 transcription factor family.
    Mol Cell Biol. 1990 Nov;10(11):5857-64 PMID: 2172787
  45. A gene activated by growth factors is related to the oncogene v-jun.
    Proc Natl Acad Sci U S A. 1988 Mar;85(5):1487-91 PMID: 3422745
  46. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.
    Science. 1988 Jan 29;239(4839):487-91 PMID: 2448875
  47. Transforming but not immortalizing oncogenes activate the transcription factor PEA1.
    EMBO J. 1988 Aug;7(8):2475-83 PMID: 3142763
  48. Phorbol ester-inducible genes contain a common cis element recognized by a TPA-modulated trans-acting factor.
    Cell. 1987 Jun 19;49(6):729-39 PMID: 3034432
  49. Requirement for fos gene expression in the transcriptional activation of collagenase by other oncogenes and phorbol esters.
    Cell. 1988 Jul 29;54(3):325-34 PMID: 2840203
  50. Prolonged activation of jun and collagenase genes by tumour necrosis factor-alpha.
    Nature. 1989 Feb 16;337(6208):661-3 PMID: 2537468
  51. Differentiation-responsive elements in the 5' region of the mouse tissue plasminogen activator gene confer two-stage regulation by retinoic acid and cyclic AMP in teratocarcinoma cells.
    Mol Cell Biol. 1989 Apr;9(4):1691-704 PMID: 2542775
  52. Multiple cis- and trans-acting elements mediate the transcriptional response to phorbol esters.
    Nature. 1987 Oct 15-21;329(6140):648-51 PMID: 2821407
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/19.3.649
Published
1991-02-11
Pages
649-55
Language
English
Region
England
NLM ID
0411011
PMCID
PMC333661
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/19.3.649 PMC Full Text Free Full Text View journal details
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