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PMID: 1827446 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Subcellular localization and sequence of sea urchin kinesin heavy chain: evidence for its association with membranes in the mitotic apparatus and interphase cytoplasm.

The Journal of cell biology ·Vol. 113 ·No. 4 ·1991-05-00 ·Pages 817-33

Wright BD, Henson JH, Wedaman KP, Willy PJ, Morand JN, Scholey JM

Abstract

Kinesin was previously immunolocalized to mitotic apparatuses (MAs) of early sea urchin blastomeres (Scholey, J.M., M.E. Porter, P.M. Grissom, and J.R. McIntosh. 1985. Nature [Lond.]. 318:483-486). Here we report evidence that this MA-associated motor protein is a conventional membrane-bound kinesin, rather than a kinesin-like protein. Our evidence includes the observation that the deduced amino acid sequence of this sea urchin kinesin heavy chain is characteristic of a conventional kinesin. In addition, immunolocalizations using antibodies that distinguish kinesin from kinesin-like proteins confirm that conventional kinesin is concentrated in MAs. Finally, our immunocytochemical data further suggest that conventional kinesin is associated with membranes which accumulate in MAs and interphase asters of early sea urchin embryos, and with vesicles that are distributed in the perinuclear region of coelomocytes. Thus kinesin may function as a microtubule-based vesicle motor in some MAs, as well as in the interphase cytoplasm.

MeSH Terms
Adenosine Triphosphatases/genetics,metabolism Amino Acid Sequence Animals Antibodies, Monoclonal/immunology Blotting, Western Calsequestrin/metabolism Cell Compartmentation Cleavage Stage, Ovum Cloning, Molecular DNA/genetics Interphase Intracellular Membranes/metabolism Kinesins Microtubules/metabolism Molecular Sequence Data Nocodazole/pharmacology Peritoneal Cavity/cytology Restriction Mapping Sea Urchins/cytology,ultrastructure Spindle Apparatus/metabolism,ultrastructure Tubulin/metabolism
Chemicals
Antibodies, Monoclonal Calsequestrin Tubulin DNA Adenosine Triphosphatases Kinesins Nocodazole
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Wright B D
Department of Zoology, University of California, Davis 95616.
Henson J H
Wedaman K P
Willy P J
Morand J N
Scholey J M
References (51)
51 references, click to expand
  1. Monoclonal antibodies to kinesin heavy and light chains stain vesicle-like structures, but not microtubules, in cultured cells.
    J Cell Biol. 1989 Apr;108(4):1453-63 PMID: 2522455
  2. One motor, many tails: an expanding repertoire of force-generating enzymes.
    Cell. 1990 Mar 23;60(6):883-5 PMID: 2138513
  3. A three-domain structure of kinesin heavy chain revealed by DNA sequence and microtubule binding analyses.
    Cell. 1989 Mar 10;56(5):879-89 PMID: 2522352
  4. Submolecular domains of bovine brain kinesin identified by electron microscopy and monoclonal antibody decoration.
    Cell. 1989 Mar 10;56(5):867-78 PMID: 2522351
  5. Isolation of a 45-kDa fragment from the kinesin heavy chain with enhanced ATPase and microtubule-binding activities.
    J Biol Chem. 1989 Jan 5;264(1):589-95 PMID: 2521221
  6. Identification of globular mechanochemical heads of kinesin.
    Nature. 1989 Mar 23;338(6213):355-7 PMID: 2493586
  7. A calsequestrin-like protein in the endoplasmic reticulum of the sea urchin: localization and dynamics in the egg and first cell cycle embryo.
    J Cell Biol. 1989 Jul;109(1):149-61 PMID: 2663877
  8. Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility.
    Cell. 1985 Aug;42(1):39-50 PMID: 3926325
  9. Molecular cloning of the microtubule-associated mechanochemical enzyme dynamin reveals homology with a new family of GTP-binding proteins.
    Nature. 1990 Sep 20;347(6290):256-61 PMID: 2144893
  10. Use of T7 RNA polymerase to direct expression of cloned genes.
    Methods Enzymol. 1990;185:60-89 PMID: 2199796
  11. Microtubules, membrane traffic, and cell organization.
    Cell. 1990 Apr 6;61(1):5-7 PMID: 2180584
  12. A kinesin-like protein required for distributive chromosome segregation in Drosophila.
    Cell. 1990 Sep 21;62(6):1053-62 PMID: 2144792
  13. Radial extension of macrophage tubular lysosomes supported by kinesin.
    Nature. 1990 Aug 30;346(6287):864-6 PMID: 1697403
  14. Mediation of meiotic and early mitotic chromosome segregation in Drosophila by a protein related to kinesin.
    Nature. 1990 May 3;345(6270):81-3 PMID: 1691829
  15. A monoclonal antibody against kinesin inhibits both anterograde and retrograde fast axonal transport in squid axoplasm.
    Proc Natl Acad Sci U S A. 1990 Feb;87(3):1061-5 PMID: 1689058
  16. Microtubule- and motor-dependent fusion in vitro between apical and basolateral endocytic vesicles from MDCK cells.
    Cell. 1990 Aug 24;62(4):719-31 PMID: 2143699
  17. Evidence that the head of kinesin is sufficient for force generation and motility in vitro.
    Science. 1990 Jul 6;249(4964):42-7 PMID: 2142332
  18. Light chains of sea urchin kinesin identified by immunoadsorption.
    Cell Motil Cytoskeleton. 1990;16(3):204-13 PMID: 2142020
  19. Identification and characterization of a gene encoding a kinesin-like protein in Drosophila.
    Cell. 1990 Jun 15;61(6):991-1000 PMID: 2140958
  20. KAR3, a kinesin-related gene required for yeast nuclear fusion.
    Cell. 1990 Mar 23;60(6):1029-41 PMID: 2138512
  21. Mutation of a gene that encodes a kinesin-like protein blocks nuclear division in A. nidulans.
    Cell. 1990 Mar 23;60(6):1019-27 PMID: 2138511
  22. The primary structure and analysis of the squid kinesin heavy chain.
    J Biol Chem. 1990 Feb 25;265(6):3278-83 PMID: 2137456
  23. A comprehensive set of sequence analysis programs for the VAX.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 PMID: 6546423
  24. Affinity purification of antibodies from diazotized paper blots of heterogeneous protein samples.
    J Biol Chem. 1981 Dec 10;256(23):11955-7 PMID: 7028745
  25. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  26. Dynamic aspects of filopodial formation by reorganization of microfilaments.
    J Cell Biol. 1977 May;73(2):479-91 PMID: 558198
  27. The role of membranes in the ogranization of the mitotic apparatus.
    Exp Cell Res. 1975 Sep;94(2):409-25 PMID: 1238267
  28. Quantitative analysis of sea urchin egg kinesin-driven microtubule motility.
    J Biol Chem. 1989 Mar 15;264(8):4290-7 PMID: 2522443
  29. Visualization of the Ca-transport system of the mitotic apparatus of sea urchin eggs with a monoclonal antibody.
    Proc Natl Acad Sci U S A. 1986 Mar;83(6):1719-22 PMID: 16593667
  30. Some structural and functional aspects of the mitotic apparatus in sea urchin embryos.
    J Cell Biol. 1962 Sep;14:475-87 PMID: 13952946
  31. Aster-associated particles in the cleavage of marine invertebrate eggs.
    Ann N Y Acad Sci. 1960 Oct 7;90:357-80 PMID: 13740117
  32. Kinesin heavy chain is essential for viability and neuromuscular functions in Drosophila, but mutants show no defects in mitosis.
    Cell. 1991 Mar 22;64(6):1093-102 PMID: 1825937
  33. Novel potential mitotic motor protein encoded by the fission yeast cut7+ gene.
    Nature. 1990 Oct 11;347(6293):563-6 PMID: 2145514
  34. Differentiation of a calsequestrin-containing endoplasmic reticulum during sea urchin oogenesis.
    Dev Biol. 1990 Dec;142(2):255-69 PMID: 2257966
  35. Generation of single-stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus.
    Proc Natl Acad Sci U S A. 1988 Oct;85(20):7652-6 PMID: 3174659
  36. GTP-binding domain: three consensus sequence elements with distinct spacing.
    Proc Natl Acad Sci U S A. 1987 Apr;84(7):1814-8 PMID: 3104905
  37. Intracellular transport using microtubule-based motors.
    Annu Rev Cell Biol. 1987;3:347-78 PMID: 3120763
  38. Kinesin is associated with a nonmicrotubule component of sea urchin mitotic spindles.
    Proc Natl Acad Sci U S A. 1987 May;84(9):2771-5 PMID: 3106977
  39. Kinesin ATPase: rate-limiting ADP release.
    Proc Natl Acad Sci U S A. 1988 Sep;85(17):6314-8 PMID: 2970638
  40. Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains.
    J Cell Biol. 1988 Dec;107(6 Pt 2):2657-67 PMID: 2974459
  41. Identification of kinesin in sea urchin eggs, and evidence for its localization in the mitotic spindle.
    Nature. 1985 Dec 5-11;318(6045):483-6 PMID: 2933590
  42. Isolation and characterization of the gene encoding the heavy chain of Drosophila kinesin.
    Proc Natl Acad Sci U S A. 1988 Mar;85(6):1864-8 PMID: 3126498
  43. Native structure and physical properties of bovine brain kinesin and identification of the ATP-binding subunit polypeptide.
    Biochemistry. 1988 May 3;27(9):3409-16 PMID: 3134048
  44. The role of kinesin and other soluble factors in organelle movement along microtubules.
    J Cell Biol. 1988 Nov;107(5):1785-92 PMID: 3141429
  45. What are the functions of kinesin?
    Bioessays. 1987 Oct;7(4):165-8 PMID: 3120706
  46. Characterization of the microtubule movement produced by sea urchin egg kinesin.
    J Biol Chem. 1987 Feb 25;262(6):2794-802 PMID: 3102475
  47. Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.
    Proc Natl Acad Sci U S A. 1988 Dec;85(23):8998-9002 PMID: 2461560
  48. Gene isolation by screening lambda gt11 libraries with antibodies.
    Methods Enzymol. 1987;152:458-69 PMID: 2443804
  49. Different axoplasmic proteins generate movement in opposite directions along microtubules in vitro.
    Cell. 1985 Dec;43(3 Pt 2):623-32 PMID: 2416467
  50. The distribution, abundance and subcellular localization of kinesin.
    J Cell Biol. 1989 Jun;108(6):2335-42 PMID: 2525563
  51. Potential roles of microtubule-associated motor molecules in cell division.
    Ann N Y Acad Sci. 1990;582:99-107 PMID: 2141453
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.113.4.817
Published
1991-05-00
Pages
817-33
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2288992
Subset
IM
Grants
PHS HHS · 20217 · United States
Databases
GENBANK
X56844
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1083/jcb.113.4.817 PMC Full Text View journal details
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