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PMID: 18337750 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The BTG2 protein is a general activator of mRNA deadenylation.

The EMBO journal ·Vol. 27 ·No. 7 ·2008-04-09 ·Pages 1039-48

Mauxion F, Faux C, Séraphin B

Abstract

BTG2 is a prototype member of the BTG/Tob family of antiproliferative proteins, originally identified as a primary response gene induced by growth factors and tumour promoters. Its expression has been linked to diverse cellular processes such as cell-cycle progression, differentiation or apoptosis. BTG2 has also been shown to interact with the Pop2/Caf1 deadenylase. Here, we demonstrate that BTG2 is a general activator of mRNA decay, thereby contributing to gene expression control. Detailed characterizations of BTG2 show that it enhances deadenylation of all transcripts tested. Our results demonstrate that Caf1 nuclease activity is required for efficient deadenylation in mammalian cells and that the deadenylase activities of both Caf1 and its Ccr4 partner are required for Btg2-induced poly(A) degradation. General activation of deadenylation may represent a new mode of global regulation of gene expression, which could be important to allow rapid resetting of protein production during development or after specific stresses. This may constitute a common function for BTG/Tob family members.

MeSH Terms
Animals Catalytic Domain Cell Line Exoribonucleases Gene Expression Regulation Genes, Dominant Genes, Reporter Genes, Tumor Suppressor Globins/metabolism Half-Life Humans Immediate-Early Proteins/metabolism Mice Mutant Proteins/metabolism Polyadenylation Protein Binding Proteins/metabolism RNA Stability RNA, Messenger/genetics,metabolism Receptors, CCR4/metabolism Repressor Proteins Ribonucleases/metabolism Transfection Tumor Suppressor Proteins
Chemicals
Immediate-Early Proteins Mutant Proteins Proteins RNA, Messenger Receptors, CCR4 Repressor Proteins Tumor Suppressor Proteins BTG2 protein, human Globins Cnot7 protein, mouse Exoribonucleases Ribonucleases mRNA deadenylase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Mauxion Fabienne
CNRS, Equipe Labellisée La Ligue, Centre de Génétique Moléculaire, UPR 2167, Gif-sur-Yvette, France. [email protected]
Faux Céline
Séraphin Bertrand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
1460-2075
DOI
10.1038/emboj.2008.43
Email
[email protected]
Published
2008-04-09
Epub
2008-00-13
Pages
1039-48
Language
English
Region
England
NLM ID
8208664
PMCID
PMC2323266
Subset
IM
External Links
PubMed source DOI: 10.1038/emboj.2008.43 PMC Full Text View journal details
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