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PMID: 1834996 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The N-terminal part of the E.coli DNA binding protein FIS is essential for stimulating site-specific DNA inversion but is not required for specific DNA binding.

Nucleic acids research ·Vol. 19 ·No. 21 ·1991-11-11 ·Pages 5915-22

Koch C, Ninnemann O, Fuss H, Kahmann R

Abstract

FIS protein is involved in several different cellular processes stimulating site-specific recombination in phages Mu and lambda as well as transcription of stable RNA operons in E.coli. We have performed a mutational analysis of fis and provide genetic and biochemical evidence that a truncated version of FIS lacking the N-terminal region is sufficient for specific DNA binding and for stimulating lambda excision. These mutants also retain their ability to autoregulate fis gene expression. Such mutant proteins, however, cannot stimulate the enhancer dependent DNA inversion reaction.

Related Genes
fis
MeSH Terms
Amino Acid Sequence Bacterial Proteins/chemistry,genetics,metabolism Bacteriophage lambda/genetics Bacteriophage mu/genetics Base Sequence Carrier Proteins/chemistry,genetics,metabolism Chromosome Inversion DNA Mutational Analysis DNA-Binding Proteins/chemistry,genetics,metabolism Enhancer Elements, Genetic/genetics Escherichia coli/genetics Escherichia coli Proteins Factor For Inversion Stimulation Protein Gene Expression Regulation, Bacterial/genetics Integration Host Factors Molecular Sequence Data Plasmids/genetics Recombination, Genetic/genetics
Chemicals
Bacterial Proteins Carrier Proteins DNA-Binding Proteins Escherichia coli Proteins Factor For Inversion Stimulation Protein Integration Host Factors integration host factor, E coli
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Koch C
Institut für Genbiologische Forschung Berlin GmbH, FRG.
Ninnemann O
Fuss H
Kahmann R
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40 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/19.21.5915
Published
1991-11-11
Pages
5915-22
Language
English
Region
England
NLM ID
0411011
PMCID
PMC329047
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/19.21.5915 PMC Full Text View journal details
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