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PMID: 3057497 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Promoters largely determine the efficiency of repressor action.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 23 ·1988-12-00 ·Pages 8973-7

Lanzer M, Bujard H

Abstract

Operator sequence and repressor protein regulate the activity of the lac promoter over a greater than 1000-fold range. Combinations of the lac operator with other promoter sequences, however, differ vastly in the level of repression. The data presented show that the extent of repression is determined largely by the rates of complex formation of the competing systems operator-repressor and promoter-RNA polymerase and by the rate at which RNA polymerase clears the promoter. Moreover, up to 70-fold differences in the level of repression were found when the operator was placed in different positions within the promoter sequence. A kinetic model is proposed that explains the observed effects and that allows predictions on promoters controlled by negatively acting elements.

MeSH Terms
Base Sequence Escherichia coli/genetics Kinetics Lac Operon Molecular Sequence Data Plasmids Promoter Regions, Genetic Repressor Proteins/metabolism Transcription Factors/metabolism Transcription, Genetic
Chemicals
Repressor Proteins Transcription Factors
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lanzer M
Zentrum für Molekulare Biologie, Universität Heidelberg, Federal Republic of Germany.
Bujard H
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20 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.23.8973
Published
1988-12-00
Pages
8973-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC282639
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.85.23.8973 PMC Full Text View journal details
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