The interaction of RNA polymerase and lac repressor with the lac control region.

Nucleic acids research ·Vol. 6 ·No. 1 ·1979-01-00 ·Pages 111-37

Schmitz A, Galas DJ

Abstract

We have examined the interactions of lac repressor and RNA polymerase with the DNA of the lac control region, using a method for direct visualization of the regions of DNA protected by proteins from DNAase attack. The repressor protects the operator essentially as reported by Gilbert and Maxam (1) with some small modifications. However, the evidence reported here concerning the binding of RNA polymerase to the DNA of the promoter mutant UV5 indicates that : 1) the RNA polymerase molecule binds asymmetrically to the promoter DNA, 2) RNA polymerase protects DNA sequences to within a few bases of the CAP binding site, suggesting direct interaction between polymerase and the CAP protein at this site, 3) RNA polymerase still binds to the promoter when repressor is bound to the operator, but fails to form the same extensive complex.

MeSH Terms

Base Sequence DNA, Bacterial/biosynthesis DNA-Directed RNA Polymerases/metabolism Escherichia coli/metabolism Lac Operon Models, Molecular Nucleic Acid Conformation Repressor Proteins/metabolism Transcription Factors/metabolism

Chemicals

DNA, Bacterial Repressor Proteins Transcription Factors DNA-Directed RNA Polymerases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Schmitz A

Galas D J

References (28)

28 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/6.1.111

Published

1979-01-00

Pages

111-37

Language

English

Region

England

NLM ID

0411011

PMCID

PMC327677

Subset

External Links

PubMed source DOI: 10.1093/nar/6.1.111 PMC Full Text View journal details

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The interaction of RNA polymerase and lac repressor with the lac control region.

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