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PMID: 18371395 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Hematopoietic fingerprints: an expression database of stem cells and their progeny.

Cell stem cell ·Vol. 1 ·No. 5 ·2007-11-00 ·Pages 578-91

Chambers SM, Boles NC, Lin KY, Tierney MP, Bowman TV, Bradfute SB, Chen AJ, Merchant AA, Sirin O, Weksberg DC, Merchant MG, Fisk CJ, Shaw CA, Goodell MA

Abstract

Hematopoietic stem cells (HSCs) continuously regenerate the hematologic system, yet few genes regulating this process have been defined. To identify candidate factors involved in differentiation and self-renewal, we have generated an expression database of hematopoietic stem cells and their differentiated progeny, including erythrocytes, granulocytes, monocytes, NK cells, activated and naive T cells, and B cells. Bioinformatic analysis revealed HSCs were more transcriptionally active than their progeny and shared a common activation mechanism with T cells. Each cell type also displayed unique biases in the regulation of particular genetic pathways, with Wnt signaling particularly enhanced in HSCs. We identified approximately 100-400 genes uniquely expressed in each cell type, termed lineage "fingerprints." In overexpression studies, two of these genes, Zfp 105 from the NK cell lineage, and Ets2 from the monocyte lineage, were able to significantly influence differentiation toward their respective lineages, demonstrating the utility of the fingerprints for identifying genes that regulate differentiation.

MeSH Terms
Animals Cell Differentiation/genetics Cell Line Cell Lineage/genetics Cell Proliferation Chromatin Assembly and Disassembly Computational Biology DNA-Binding Proteins/genetics Databases, Genetic Female Gene Expression Profiling Gene Expression Regulation Genotype Hematopoietic Stem Cells/physiology Mice Mice, Inbred C57BL Phenotype Proto-Oncogene Protein c-ets-2/genetics Transcription, Genetic Wnt Proteins/genetics
Chemicals
DNA-Binding Proteins Ets2 protein, mouse Proto-Oncogene Protein c-ets-2 Wnt Proteins Zfp 105 protein, mouse
Authors & Affiliations
14 authors, click to expand affiliations / ORCID
Chambers Stuart M
Interdepartmental Program of Cell and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA.
Boles Nathan C
Lin Kuan-Yin K
Tierney Megan P
Bowman Teresa V
Bradfute Steven B
Chen Alice J
Merchant Akil A
Sirin Olga
Weksberg David C
Merchant Mehveen G
Fisk C Joseph
Shaw Chad A
Goodell Margaret A
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Article Info
Journal
Cell stem cell
Abbr.
Cell Stem Cell
ISSN
1934-5909
DOI
10.1016/j.stem.2007.10.003
Published
2007-11-00
Pages
578-91
Language
English
Region
United States
NLM ID
101311472
PMCID
PMC2475548
Subset
IM
Grants
NHLBI NIH HHS · T32 HL092332-06 · United States
NIDDK NIH HHS · DK63588 · United States
NIDDK NIH HHS · T32 DK064717-05 · United States
NIA NIH HHS · T32 AG000183 · United States
NIDDK NIH HHS · DK58192 · United States
NHLBI NIH HHS · T32 HL092332 · United States
NIDDK NIH HHS · U01 DK063588-03 · United States
NCI NIH HHS · P50 CA126752-01 · United States
NIAID NIH HHS · T32 AI007495 · United States
NIDDK NIH HHS · U01 DK063588 · United States
NIA NIH HHS · T32 AG000183-13 · United States
NIDDK NIH HHS · R01 DK058192 · United States
NIDDK NIH HHS · R01 DK058192-06 · United States
NIAID NIH HHS · T32 AI07495 · United States
NCI NIH HHS · P50 CA126752 · United States
NIDDK NIH HHS · T32 DK064717 · United States
External Links
PubMed source DOI: 10.1016/j.stem.2007.10.003 PMC Full Text View journal details
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