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PMID: 1840911 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

A 3' stem/loop structure of the Chlamydomonas chloroplast atpB gene regulates mRNA accumulation in vivo.

The Plant cell ·Vol. 3 ·No. 3 ·1991-03-00 ·Pages 285-97

Stern DB, Radwanski ER, Kindle KL

Abstract

The Chlamydomonas reinhardtii chloroplast atpB mRNA contains sequences at its 3' end that can form a complex stem/loop structure. Deletions of part or all of this sequence in transformed C. reinhardtii cells led to decreased atpB mRNA accumulation, whereas transcription rates were unaffected. The reduction of mRNA to 20% to 35% of wild-type levels in transformants without 3' stem/loops was correlated with the accumulation of atpB mRNA that was heterogeneous in size. These results indicated that RNA secondary structures function both in mRNA stabilization and in 3' end formation in C. reinhardtii chloroplasts. Furthermore, deletion of the stem/loop resulted in a decrease in the steady-state level of the ATPase beta-subunit to approximately 60% of wild-type levels, suggesting that translational and/or post-translational mechanisms may influence the steady-state level of the atpB gene product.

Related Genes
atpB petD psbA rpoA
MeSH Terms
Adenosine Triphosphatases/genetics Animals Base Sequence Chlamydomonas/genetics Chloroplasts Genes, Plant Genes, Protozoan Molecular Sequence Data Nucleic Acid Conformation Plant Proteins/genetics Plants/genetics Protozoan Proteins/genetics RNA, Messenger/metabolism RNA, Protozoan/metabolism Repetitive Sequences, Nucleic Acid Transcription, Genetic
Chemicals
Plant Proteins Protozoan Proteins RNA, Messenger RNA, Protozoan Adenosine Triphosphatases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Stern D B
Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, New York 14853.
Radwanski E R
Kindle K L
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.3.3.285
Published
1991-03-00
Pages
285-97
Language
English
Region
England
NLM ID
9208688
PMCID
PMC159999
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.3.3.285 PMC Full Text View journal details
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