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PMID: 18425677 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Radiofrequency thermal ablation of breast tumors combined with intralesional administration of IL-7 and IL-15 augments anti-tumor immune responses and inhibits tumor development and metastasis.

Breast cancer research and treatment ·Vol. 114 ·No. 3 ·2009-04-00 ·Pages 423-31

Habibi M, Kmieciak M, Graham L, Morales JK, Bear HD, Manjili MH

Abstract

Tumor development or recurrence is always a matter of concern following radiofrequency thermal ablation (RFA) of tumors. To determine whether combining RFA with immunologically active cytokines might induce tumor-specific immune responses against mammary carcinoma and inhibit tumor development or metastasis, we evaluated intralesional injection of IL-7 and IL-15 in RFA-treated murine tumors. We used two different breast carcinoma models: neu-overexpressing mouse mammary carcinoma (MMC) in FVBN202 transgenic mouse and 4T1 tumors in Balb/c mouse. MMC tend to relapse even in the presence of neu-specific immune responses, and 4T1 is a weakly immunogenic, aggressive and highly metastatic transplantable tumor. In vivo growth of both of these tumors is also associated with increased numbers of CD11b+Gr1+ myeloid-derived suppressor cells (MDSC). We showed for the first time that unlike RFA alone, RFA combined with the administration of intralesional IL-7 and IL-15 (after RFA), induced immune responses to tumors, inhibited tumor development and lung metastasis, and reduced MDSC.

MeSH Terms
Animals Antineoplastic Agents/therapeutic use Catheter Ablation/methods Female Immune System Immunotherapy/methods Interferon-gamma/metabolism Interleukin-15/therapeutic use Interleukin-7/therapeutic use Mammary Neoplasms, Animal/therapy Mice Mice, Inbred BALB C Mice, Transgenic Neoplasm Metastasis Rats
Chemicals
Antineoplastic Agents Interleukin-15 Interleukin-7 Interferon-gamma
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Habibi Mehran
Department of Microbiology and Immunology, VCU School of Medicine, Massey Cancer Center, 401 College Street, Box 980035, Richmond, VA 23298, USA.
Kmieciak Maciej
Graham Laura
Morales Johanna K
Bear Harry D
Manjili Masoud H
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Article Info
Journal
Breast cancer research and treatment
Abbr.
Breast Cancer Res Treat
ISSN
1573-7217
DOI
10.1007/s10549-008-0024-3
Published
2009-04-00
Epub
2008-00-20
Pages
423-31
Language
English
Region
Netherlands
NLM ID
8111104
PMCID
PMC2649692
Subset
IM
Grants
NCI NIH HHS · P30 CA016059 · United States
NCI NIH HHS · R01 CA104757 · United States
NCI NIH HHS · R01 CA104757-03 · United States
NCI NIH HHS · P30CA16059 · United States
External Links
PubMed source DOI: 10.1007/s10549-008-0024-3 PMC Full Text View journal details
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