Home LiteratureArticle Details
PMID: 1844573 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Coinjection strategy for visual identification of transgenic mice.

Transgenic research ·Vol. 1 ·No. 1 ·1991-12-00 ·Pages 31-7

Overbeek PA, Aguilar-Cordova E, Hanten G, Schaffner DL, Patel P, Lebovitz RM, Lieberman MW

Abstract

Transgenic mice were generated by coinjection of a dominant marker gene that induces fur and eye pigmentation (a tyrosinase minigene) plus an unrelated DNA construction that has a gamma-glutamyl transferase (gamma GT) promoter linked to a ras oncogene. Mice transgenic for gamma GT-ras could be identified in the first and all subsequent generations by simple visual inspection for pigmentation. Furthermore, the gamma-glutamyl transferase promoter was active in kidney but not skin of the transgenic mice, indicating that the cointegrated DNA was active and independently expressed. These results confirm that the tyrosinase minigene can be used for coinjections to allow rapid visual identification of transgenic mice.

Related Genes
ras
MeSH Terms
Animals Base Sequence Crosses, Genetic DNA/genetics,isolation & purification Female Genes, ras Genetic Techniques Hair Color/genetics Male Mice Mice, Inbred Strains Mice, Transgenic Molecular Sequence Data Oligodeoxyribonucleotides Pedigree Polymerase Chain Reaction/methods Restriction Mapping
Chemicals
Oligodeoxyribonucleotides DNA
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Overbeek P A
Howard Hughes Medical Institute, Department of Cell Biology, Baylor College of Medicine, Houston, TX 77030.
Aguilar-Cordova E
Hanten G
Schaffner D L
Patel P
Lebovitz R M
Lieberman M W
References (21)
21 references, click to expand
  1. Cloning and analysis of the rat gamma-glutamyltransferase gene.
    J Biol Chem. 1990 Jul 15;265(20):11721-5 PMID: 1973164
  2. Synthesis of functional human hemoglobin in transgenic mice.
    Science. 1989 Sep 1;245(4921):971-3 PMID: 2772649
  3. Somatic expression of herpes thymidine kinase in mice following injection of a fusion gene into eggs.
    Cell. 1981 Nov;27(1 Pt 2):223-31 PMID: 6276022
  4. FVB/N: an inbred mouse strain preferable for transgenic analyses.
    Proc Natl Acad Sci U S A. 1991 Mar 15;88(6):2065-9 PMID: 1848692
  5. Mechanism of chromosomal integration of microinjected DNA.
    Mol Biol Med. 1989 Aug;6(4):283-98 PMID: 2695741
  6. Studies of gamma-glutamyl transpeptidase in human ocular tissues.
    Exp Eye Res. 1976 Apr;22(4):329-34 PMID: 8323
  7. Complete nucleotide sequences of the T24 human bladder carcinoma oncogene and its normal homologue.
    Nature. 1983 Mar 3;302(5903):33-7 PMID: 6298635
  8. Human sickle hemoglobin in transgenic mice.
    Science. 1990 Feb 2;247(4942):566-8 PMID: 2154033
  9. Regulation of a metallothionein-rasT24 fusion gene by zinc results in graded alterations in cell morphology and growth.
    Oncogene. 1987;1(3):323-30 PMID: 3330778
  10. Altered immunoglobulin expression and functional silencing of self-reactive B lymphocytes in transgenic mice.
    Nature. 1988 Aug 25;334(6184):676-82 PMID: 3261841
  11. Tyrosinase as a marker for transgenic mice.
    Nucleic Acids Res. 1991 Feb 25;19(4):958 PMID: 1673234
  12. Expression in transgenic mice of two genes of different tissue specificity integrated into a single chromosomal site.
    Genes Dev. 1987 Dec;1(10):1075-84 PMID: 3123313
  13. Melanization in albino mice transformed by introducing cloned mouse tyrosinase gene.
    Development. 1990 Feb;108(2):223-7 PMID: 2112453
  14. Bovine lens -glutamyl transpeptidase.
    Exp Eye Res. 1973 Feb;15(2):161-71 PMID: 4144220
  15. Human gamma- to beta-globin gene switching in transgenic mice.
    Genes Dev. 1990 Mar;4(3):380-9 PMID: 1692558
  16. Transgenic mice with mu and kappa genes encoding antiphosphorylcholine antibodies.
    J Exp Med. 1986 Aug 1;164(2):627-41 PMID: 2425036
  17. Rescue of the albino phenotype by introduction of a functional tyrosinase gene into mice.
    EMBO J. 1990 Sep;9(9):2819-26 PMID: 2118105
  18. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.
    J Mol Appl Genet. 1982;1(4):327-41 PMID: 6286831
  19. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
    Anal Biochem. 1987 Apr;162(1):156-9 PMID: 2440339
  20. Conserved cysteine to serine mutation in tyrosinase is responsible for the classical albino mutation in laboratory mice.
    Nucleic Acids Res. 1990 Dec 25;18(24):7293-8 PMID: 2124349
  21. Activation of the T24 bladder carcinoma transforming gene is linked to a single amino acid change.
    Nature. 1982 Dec 23;300(5894):762-5 PMID: 7177195
Article Info
Journal
Transgenic research
Abbr.
Transgenic Res
ISSN
0962-8819
DOI
10.1007/BF02512994
Published
1991-12-00
Pages
31-7
Language
English
Region
Netherlands
NLM ID
9209120
Subset
IM
Grants
NCI NIH HHS · CA40263 · United States
NICHD NIH HHS · HD25340 · United States
External Links
PubMed source DOI: 10.1007/BF02512994 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2