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PMID: 1846032 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The Ams (altered mRNA stability) protein and ribonuclease E are encoded by the same structural gene of Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 1 ·1991-01-01 ·Pages 1-5

Babitzke P, Kushner SR

Abstract

The in vitro and in vivo analysis of the ribonuclease E-deficient (rne-) and the altered mRNA stability protein-deficient (ams-) strains of Escherichia coli has demonstrated that they carry mutations in the same structural gene. Strains encoding either thermolabile RNase E (rne-3071) or Ams protein (ams-1) are defective in both rRNA processing and mRNA turnover. Immediately after a shift to the nonpermissive temperature, the chemical decay rate of bulk mRNA is slowed 2- to 3-fold, and within 70 min, precursors to 5S rRNA begin to accumulate. In addition, all of the phenotypes associated with either the rne-3071 or the ams-1 alleles were complemented by a recombinant plasmid carrying ams+. When taken together with previous genetic studies, these results suggest that the role of ribonuclease E in mRNA turnover involves endonucleolytic cleavages at the proposed ACAG(A/U)AUUUG consensus sequence.

Related Genes
ams rne
MeSH Terms
Bacterial Proteins/genetics,metabolism Base Sequence Chromosome Deletion DNA Transposable Elements Endoribonucleases/genetics,metabolism Escherichia coli/enzymology,genetics Genes, Bacterial Genetic Complementation Test Genotype Kinetics Molecular Sequence Data Operon RNA, Messenger/metabolism
Chemicals
Bacterial Proteins DNA Transposable Elements RNA, Messenger Endoribonucleases ribonuclease E
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Babitzke P
Department of Genetics, University of Georgia, Athens 30602.
Kushner S R
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.1.1
Published
1991-01-01
Pages
1-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC50735
Subset
IM
Grants
NIGMS NIH HHS · GM07103 · United States
NIGMS NIH HHS · GM28760 · United States
External Links
PubMed source DOI: 10.1073/pnas.88.1.1 PMC Full Text View journal details
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