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PMID: 2417233 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Polynucleotide phosphorylase and ribonuclease II are required for cell viability and mRNA turnover in Escherichia coli K-12.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 1 ·1986-01-00 ·Pages 120-4

Donovan WP, Kushner SR

Abstract

The isolation of a temperature-sensitive allele of RNase II (rnb) by in vitro mutagenesis has permitted the demonstration that RNase II and polynucleotide phosphorylase (PNPase) are required for cell viability and mRNA turnover in Escherichia coli. Double-mutant strains carrying the pnp-7 and rnb-500 alleles (PNPase deficient and RNase II thermolabile) ceased growing in Luria broth within 30 min after shift to the nonpermissive temperature. Cessation of growth was accompanied by an accumulation of mRNA fragments 100-1500 nucleotides long. In contrast, single-mutant and wild-type control strains grew normally at the nonpermissive temperature and did not accumulate mRNA. No significant changes in rRNA patterns were observed in any of the strains.

MeSH Terms
Drug Stability Escherichia coli/enzymology,genetics,growth & development Exoribonucleases/genetics,physiology Genes, Bacterial Half-Life Hot Temperature Kinetics Mutation Nucleic Acid Hybridization Polyribonucleotide Nucleotidyltransferase/genetics,physiology RNA, Bacterial/metabolism RNA, Messenger/metabolism
Chemicals
RNA, Bacterial RNA, Messenger Polyribonucleotide Nucleotidyltransferase Exoribonucleases exoribonuclease II
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Donovan W P
Kushner S R
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21 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.1.120
Published
1986-01-00
Pages
120-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC322803
Subset
IM
Grants
NIGMS NIH HHS · GM27997 · United States
NIGMS NIH HHS · GM28760 · United States
External Links
PubMed source DOI: 10.1073/pnas.83.1.120 PMC Full Text View journal details
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