Home LiteratureArticle Details
PMID: 6338477 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Amplification of ribonuclease II (rnb) activity in Escherichia coli K-12.

Nucleic acids research ·Vol. 11 ·No. 2 ·1983-01-25 ·Pages 265-75

Donovan WP, Kushner SR

Abstract

A 7.1 kb HindIII-XhoI fragment of E. coli DNA which contains the structural gene for ribonuclease II (rnb) has been cloned in the recombinant plasmid pDK24. At least two constitutively expressed genes are encoded on the fragment as shown by maxicell analysis. On denaturing polyacrylamide gels RNase II appears as a single 72,000 dalton species. The approximate site of transcription initiation of the rnb gene has been mapped. Although derivatives of E. coli harboring pDK24 contained 10-fold more RNase II activity that wild type strains without the plasmid, the degradation rate of mRNA was similar in all strains tested. Strains deficient in both RNase II and polynucleotide phosphorylase appear inviable.

MeSH Terms
Cloning, Molecular DNA, Recombinant/metabolism Escherichia coli/enzymology,genetics Exoribonucleases/genetics Gene Amplification Genes Genes, Bacterial Kinetics Molecular Weight Plasmids Transcription, Genetic
Chemicals
DNA, Recombinant Exoribonucleases exoribonuclease II
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Donovan W P
Kushner S R
References (27)
27 references, click to expand
  1. The involvement of ribonuclease I, ribonuclease II, and polynucleotide phosphorylase in the degradation of stable ribonucleic acid during carbon starvation in Escherichia coli.
    J Biol Chem. 1974 Jan 10;249(1):149-51 PMID: 4358625
  2. Degradation of RNA in Escherichia coli. A hypothesis.
    Mol Gen Genet. 1973 May 28;122(4):313-22 PMID: 4577538
  3. Purification and some novel properties of Escherichia coli RNase II.
    J Biol Chem. 1977 Dec 25;252(24):8945-9 PMID: 336625
  4. Apparent involvement of ribonuclease D in the 3' processing of tRNA precursors.
    Proc Natl Acad Sci U S A. 1980 Feb;77(2):837-41 PMID: 6153805
  5. The processive degradation of individual polyribonucleotide chains. I. Escherichia coli ribonuclease II.
    J Biol Chem. 1968 Mar 10;243(5):913-22 PMID: 4867942
  6. Some properties of the polynucleotide phosphorylase and ribonuclease II of Escherichia coli 1113B.
    Biochem Biophys Res Commun. 1968 Aug 21;32(4):715-22 PMID: 4300763
  7. A mutation affecting degradation of stable RNA in Escherichia coli.
    FEBS Lett. 1972 Apr 1;21(3):286-288 PMID: 11946528
  8. Models for decay of Escherichia coli lac messenger RNA and evidence for inactivating cleavages between its messages.
    J Mol Biol. 1979 Dec 5;135(2):369-90 PMID: 94103
  9. Linkage map of Escherichia coli K-12, edition 6.
    Microbiol Rev. 1980 Mar;44(1):1-56 PMID: 6997720
  10. Metabolism of messenger RNA from the gal operon of Escherichia coli.
    J Mol Biol. 1974 Dec 15;90(3):581-99 PMID: 4615171
  11. An Escherichia coli mutant with increased messenger ribonuclease activity.
    Proc Natl Acad Sci U S A. 1971 Dec;68(12):3140-4 PMID: 4943555
  12. Polynucleotide phosphorylase can participate in decay of mRNA in Escherichia coli in the absence of ribonuclease II.
    Mol Gen Genet. 1975 Sep 8;139(4):357-62 PMID: 1102947
  13. Escherichia coli mutants deficient in exoribonucleases.
    Biochem Biophys Res Commun. 1976 Jun 7;70(3):920-4 PMID: 779788
  14. Beakdown of messenger ribonucleic acid by a potassium-activated phosphodiesterase from Escherichia coli.
    J Biol Chem. 1963 Jun;238:2251-3 PMID: 13978492
  15. Purification and properties of a potassium-activated phosphodiesterase (RNAase II) from Escherichia coli.
    Biochemistry. 1965 Jul;4(7):1319-30 PMID: 5323137
  16. Simple method for identification of plasmid-coded proteins.
    J Bacteriol. 1979 Jan;137(1):692-3 PMID: 368040
  17. Characterization of polynucleotide phosphorylase mutants of Escherichia coli.
    J Bacteriol. 1969 Mar;97(3):1437-43 PMID: 4887520
  18. Increased inactivation of messengers in Escherichia coli ts mutant.
    Nat New Biol. 1972 Sep 20;239(90):79-81 PMID: 4573691
  19. Purification of RNAase II by preparative polyacrylamide gel electrophoresis.
    Biochim Biophys Acta. 1978 Feb 16;517(2):419-28 PMID: 341984
  20. Genetic analysis of an Escherichia coli syndrome.
    J Bacteriol. 1971 Dec;108(3):1322-8 PMID: 4945197
  21. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  22. Decay of ribosomal ribonucleic acid in Escherichia coli cells starved for various nutrients.
    J Biol Chem. 1975 Apr 25;250(8):3174-8 PMID: 1091648
  23. Isolation and mapping of polynucleotide phosphorylase mutants of Escherichia coli.
    J Bacteriol. 1969 Mar;97(3):1431-6 PMID: 4887519
  24. A correlation between ribonuclease II and the in vivo inactivation of messenger RNA in E. coli.
    Biochem Biophys Res Commun. 1968 Nov 8;33(3):412-7 PMID: 4881054
  25. Genetic analysis of mutations affecting ribonuclease II in Escherichia coli.
    Mol Gen Genet. 1977 May 20;153(1):1-4 PMID: 329098
  26. Evidence for endonucleolytic attack in decay of lac messenger RNA in Escherichia coli.
    J Mol Biol. 1974 Feb 25;83(2):143-61 PMID: 4595422
  27. KINETICS OF INDUCED ENZYME SYNTHESIS. DETERMINATION OF THE MEAN LIFE OF GALACTOSIDASE-SPECIFIC MESSENGER RNA.
    Biochim Biophys Acta. 1963 Oct 15;76:293-309 PMID: 14097386
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.2.265
Published
1983-01-25
Pages
265-75
Language
English
Region
England
NLM ID
0411011
PMCID
PMC325713
Subset
IM
Grants
NIGMS NIH HHS · GM27994 · United States
NIGMS NIH HHS · GM28760 · United States
External Links
PubMed source DOI: 10.1093/nar/11.2.265 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2