Home LiteratureArticle Details
PMID: 1846864 Published · ppublish English Journal Article

Polypeptides p40, pOM2, and pAngR are required for iron uptake and for virulence of the marine fish pathogen Vibrio anguillarum 775.

Journal of bacteriology ·Vol. 173 ·No. 3 ·1991-02-00 ·Pages 1347-52

Singer JT, Schmidt KA, Reno PW

Abstract

Insertions were created in three iron uptake genes in plasmid pJM1 of Vibrio anguillarum 775 to assess their in vivo effects on virulence in fish. Insertions that blocked p40, pOM2, and pAngR expression resulted in iron uptake-negative strains and in 4.2 x 10(5)-, 8.8 x 10(5)-, and 2.5 x 10(5)-fold attenuations in virulence, respectively. A strain with an insertion in the pAngR coding region still synthesized significant constitutive levels of the outer membrane protein pOM2 and persisted in fish for at least 14 days postinjection. The results demonstrate a direct relationship between virulence and three pJM1-encoded gene products and also the feasibility of constructing live attenuated strains of V. anguillarum that might be useful in future vaccines.

MeSH Terms
Bacterial Outer Membrane Proteins/genetics,metabolism DNA Transposable Elements Gene Expression Regulation, Bacterial Genes, Bacterial Iron/metabolism Plasmids Restriction Mapping Vibrio/genetics,metabolism,pathogenicity Virulence
Chemicals
Bacterial Outer Membrane Proteins DNA Transposable Elements Iron
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Singer J T
Department of Biochemistry, Microbiology, and Molecular Biology, University of Maine, Orono 04469.
Schmidt K A
Reno P W
References (27)
27 references, click to expand
  1. Evidence for the role of a siderophore in promoting Vibrio anguillarum infections.
    J Gen Microbiol. 1986 Oct;132(10):2949-52 PMID: 2957462
  2. Increased production of the siderophore anguibactin mediated by pJM1-like plasmids in Vibrio anguillarum.
    Infect Immun. 1988 Jun;56(6):1608-14 PMID: 3372022
  3. Plasmids mediating iron uptake in Vibrio anguillarum strains isolated from turbot in Spain.
    J Gen Microbiol. 1985 Aug;131(8):1989-97 PMID: 2997366
  4. Characterization of anguibactin, a novel siderophore from Vibrio anguillarum 775(pJM1).
    J Bacteriol. 1986 Jul;167(1):57-65 PMID: 3013839
  5. Plasmid-mediated iron sequestering systems in pathogenic strains of Vibrio anguillarum and Escherichia coli.
    Basic Life Sci. 1985;30:759-74 PMID: 2990437
  6. Monoclonal antibodies to infectious pancreatic necrosis virus: analysis of viral epitopes and comparison of different isolates.
    J Gen Virol. 1986 Oct;67 ( Pt 10):2193-205 PMID: 2428927
  7. Identification of polypeptides encoded by cloned pJM1 iron uptake DNA isolated from Vibrio anguillarum 775.
    J Bacteriol. 1989 May;171(5):2293-302 PMID: 2651396
  8. Relationships between plasmids and phenotypes of presumptive strains of Vibrio anguillarum isolated from different fish species.
    Appl Environ Microbiol. 1989 Apr;55(4):826-31 PMID: 2729984
  9. Molecular cloning and expression of genetic determinants for the iron uptake system mediated by the Vibrio anguillarum plasmid pJM1.
    J Bacteriol. 1984 Dec;160(3):860-6 PMID: 6094511
  10. Iron-regulated outer membrane protein OM2 of Vibrio anguillarum is encoded by virulence plasmid pJM1.
    J Bacteriol. 1985 Feb;161(2):736-42 PMID: 3968037
  11. A regulatory gene, angR, of the iron uptake system of Vibrio anguillarum: similarity with phage P22 cro and regulation by iron.
    Gene. 1990 Jan 31;86(1):45-51 PMID: 2311935
  12. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
    Gene. 1982 Oct;19(3):259-68 PMID: 6295879
  13. Nucleotide sequence of the kanamycin resistance transposon Tn903.
    J Mol Biol. 1981 Apr 5;147(2):217-26 PMID: 6270337
  14. Curing of a plasmid is correlated with an attenuation of virulence in the marine fish pathogen Vibrio anguillarum.
    Infect Immun. 1980 Mar;27(3):897-902 PMID: 6247282
  15. Iron uptake system medicated by Vibrio anguillarum plasmid pJM1.
    J Bacteriol. 1983 Nov;156(2):880-7 PMID: 6313622
  16. Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7347-51 PMID: 7012838
  17. Outer membrane proteins induced under conditions of iron limitation in the marine fish pathogen Vibrio anguillarum 775.
    Infect Immun. 1981 Jan;31(1):223-7 PMID: 7194320
  18. A plasmid associated with virulence in the marine fish pathogen Vibrio anguillarum specifies an iron-sequestering system.
    Nature. 1980 Apr 10;284(5756):566-8 PMID: 7366725
  19. Evidence for plasmid contribution to the virulence of fish pathogen Vibrio anguillarum.
    Infect Immun. 1977 Nov;18(2):509-13 PMID: 924679
  20. Solubilization of the cytoplasmic membrane of Escherichia coli by the ionic detergent sodium-lauryl sarcosinate.
    J Bacteriol. 1973 Sep;115(3):717-22 PMID: 4580564
  21. High resolution two-dimensional electrophoresis of proteins.
    J Biol Chem. 1975 May 25;250(10):4007-21 PMID: 236308
  22. A complementation analysis of the restriction and modification of DNA in Escherichia coli.
    J Mol Biol. 1969 May 14;41(3):459-72 PMID: 4896022
  23. Protection of rainbow trout against vibriosis and furunculosis by the use of attenuated strains of Vibrio anguillarum.
    Appl Environ Microbiol. 1989 Jun;55(6):1400-5 PMID: 2548446
  24. Regulation of the iron uptake system in Vibrio anguillarum: evidence for a cooperative effect between two transcriptional activators.
    Proc Natl Acad Sci U S A. 1989 May;86(10):3529-33 PMID: 2542936
  25. Genetic analysis of the iron uptake region of the Vibrio anguillarum plasmid pJM1: molecular cloning of genetic determinants encoding a novel trans activator of siderophore biosynthesis.
    J Bacteriol. 1988 Apr;170(4):1913-9 PMID: 2832388
  26. Genetics and molecular biology of siderophore-mediated iron transport in bacteria.
    Microbiol Rev. 1989 Dec;53(4):517-30 PMID: 2531838
  27. Genetic and molecular characterization of essential components of the Vibrio anguillarum plasmid-mediated iron-transport system.
    J Biol Chem. 1988 Feb 25;263(6):2853-60 PMID: 2830268
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.173.3.1347-1352.1991
Published
1991-02-00
Pages
1347-52
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC207263
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.173.3.1347-1352.1991 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2