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PMID: 2542936 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Regulation of the iron uptake system in Vibrio anguillarum: evidence for a cooperative effect between two transcriptional activators.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 10 ·1989-05-00 ·Pages 3529-33

Salinas PC, Tolmasky ME, Crosa JH

Abstract

We have identified a 110-kDa polypeptide that has a trans-acting regulatory activity on the expression of the pJM1 plasmid iron-uptake genes in Vibrio anguillarum. This protein is encoded by the angR gene and maps in a 3.6-kilobase-pair pJM1 DNA region located downstream of the iron transport genes. Full expression of this gene occurs under iron-limiting conditions and requires a 2.9-kilobase-pair upstream region in cis that maps within the coding region of the OM2 outer membrane protein, essential for the transport of iron into the cell cytosol. Determination of the siderophore anguibactin levels as well as analysis of specific transcripts for anguibactin biosynthetic genes demonstrated that AngR and another transcriptional activator, Taf, regulate in a synergistic fashion the level of anguibactin production by activation of transcription of the anguibactin biosynthetic genes under iron-limiting conditions.

MeSH Terms
Bacterial Proteins/genetics Biological Transport Blotting, Northern Cloning, Molecular DNA Mutational Analysis DNA Transposable Elements Gene Expression Regulation Genes, Bacterial Genes, Regulator Genetic Complementation Test Iron/metabolism Iron Chelating Agents Peptides Plasmids Restriction Mapping Siderophores Transcription Factors/genetics Vibrio/genetics
Chemicals
Bacterial Proteins DNA Transposable Elements Iron Chelating Agents Peptides Siderophores Transcription Factors anguibactin Iron
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Salinas P C
Department of Microbiology and Immunology, School of Medicine, Oregon Health Sciences University, Portland 97201.
Tolmasky M E
Crosa J H
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.10.3529
Published
1989-05-00
Pages
3529-33
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC287171
Subset
IM
Grants
NIAID NIH HHS · AI-19018 · United States
FIC NIH HHS · F05 TW03345-01 · United States
External Links
PubMed source DOI: 10.1073/pnas.86.10.3529 PMC Full Text View journal details
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