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PMID: 18474635 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

NGF inhibits M/KCNQ currents and selectively alters neuronal excitability in subsets of sympathetic neurons depending on their M/KCNQ current background.

The Journal of general physiology ·Vol. 131 ·No. 6 ·2008-06-00 ·Pages 575-87

Jia Z, Bei J, Rodat-Despoix L, Liu B, Jia Q, Delmas P, Zhang H

Abstract

M/KCNQ currents play a critical role in the determination of neuronal excitability. Many neurotransmitters and peptides modulate M/KCNQ current and neuronal excitability through their G protein-coupled receptors. Nerve growth factor (NGF) activates its receptor, a member of receptor tyrosine kinase (RTK) superfamily, and crucially modulates neuronal cell survival, proliferation, and differentiation. In this study, we studied the effect of NGF on the neuronal (rat superior cervical ganglion, SCG) M/KCNQ currents and excitability. As reported before, subpopulation SCG neurons with distinct firing properties could be classified into tonic, phasic-1, and phasic-2 neurons. NGF inhibited M/KCNQ currents by similar proportion in all three classes of SCG neurons but increased the excitability only significantly in tonic SCG neurons. The effect of NGF on excitability correlated with a smaller M-current density in tonic neurons. The present study indicates that NGF is an M/KCNQ channel modulator and the characteristic modulation of the neuronal excitability by NGF may have important physiological implications.

MeSH Terms
Action Potentials/drug effects,physiology Animals Cells, Cultured Electric Conductivity Electrophysiology Ion Channel Gating/drug effects Ion Transport/drug effects KCNQ Potassium Channels/drug effects,metabolism Nerve Growth Factor/pharmacology Nerve Tissue Proteins/drug effects,metabolism Neural Inhibition/drug effects,physiology Patch-Clamp Techniques Rats Rats, Sprague-Dawley Superior Cervical Ganglion/drug effects,metabolism Synaptic Transmission/drug effects,physiology Time Factors
Chemicals
KCNQ Potassium Channels Nerve Tissue Proteins Nerve Growth Factor
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Jia Zhanfeng
Department of Pharmacology, Hebei Medical University, Shijiazhuang, China 050017.
Bei Junjie
Rodat-Despoix Lise
Liu Boyi
Jia Qingzhong
Delmas Patrick
Zhang Hailin
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Article Info
Journal
The Journal of general physiology
Abbr.
J Gen Physiol
ISSN
1540-7748
DOI
10.1085/jgp.200709924
Published
2008-06-00
Epub
2008-00-12
Pages
575-87
Language
English
Region
United States
NLM ID
2985110R
PMCID
PMC2391251
Subset
IM
External Links
PubMed source DOI: 10.1085/jgp.200709924 PMC Full Text View journal details
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