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PMID: 1848314 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nuclear factor 1 activates the feline leukemia virus long terminal repeat but is posttranscriptionally down-regulated in leukemia cell lines.

Journal of virology ·Vol. 65 ·No. 4 ·1991-04-00 ·Pages 1991-9

Plumb M, Fulton R, Breimer L, Stewart M, Willison K, Neil JC

Abstract

A recombinant feline leukemia virus (FeLV) proviral clone (T17T-22) with a long terminal repeat (LTR) which differs from prototype FeLV by a point mutation within a conserved nuclear factor 1 (NF1)-binding motif in the LTR enhancer domain was found to be poorly expressed after DNA transfection. The NF1 point mutation reduced in vitro protein binding as assessed by gel shift analysis and reduced promoter activity significantly (2- to 10-fold). However, the degree of promoter impairment due to the NF1 site mutation varied according to cell type and was least severe in a feline leukemia cell line (T3) which had low levels of nuclear NF1 DNA-binding activity. Low NF1 DNA-binding activity was observed in three FeLV-induced leukemia cell lines (T3, T17, and FL74) and in murine F9 embryonal carcinoma cells. While similar levels of NF1 gene mRNA transcripts were detected in all cell lines, Western immunoblot analysis of F9, T17, and FL74 but not T3 nuclear extracts revealed very low levels of nuclear NF1 protein. These results indicate that NF1 activity is down-regulated in FeLV-induced leukemia cells by diverse posttranscriptional mechanisms. We suggest that NF1 down-regulation may be an important characteristic of target cells susceptible to FeLV transformation in vivo and may provide the selective pressure which favors duplication of the LTR core enhancer sequence in T-cell leukemogenic FeLV variants.

MeSH Terms
Animals Base Sequence CCAAT-Enhancer-Binding Proteins Cats DNA, Viral/metabolism DNA-Binding Proteins/genetics,metabolism Down-Regulation/genetics Enhancer Elements, Genetic Gene Expression Regulation Leukemia Virus, Feline/genetics Molecular Sequence Data Mutation NFI Transcription Factors Nuclear Proteins Promoter Regions, Genetic Proviruses/genetics RNA Processing, Post-Transcriptional RNA, Messenger/metabolism Repetitive Sequences, Nucleic Acid Teratoma/genetics,microbiology Transcription Factors Tumor Cells, Cultured Y-Box-Binding Protein 1
Chemicals
CCAAT-Enhancer-Binding Proteins DNA, Viral DNA-Binding Proteins NFI Transcription Factors Nuclear Proteins RNA, Messenger Transcription Factors Y-Box-Binding Protein 1 YBX1 protein, human
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Plumb M
Beatson Institute for Cancer Research, Glasgow, Scotland.
Fulton R
Breimer L
Stewart M
Willison K
Neil J C
References (51)
51 references, click to expand
  1. Disease specificity of nondefective Friend and Moloney murine leukemia viruses is controlled by a small number of nucleotides.
    J Virol. 1987 Mar;61(3):693-700 PMID: 3468264
  2. Identification of three sequence-specific DNA-binding proteins which interact with the Rous sarcoma virus enhancer and upstream promoter elements.
    J Virol. 1988 Jun;62(6):2186-90 PMID: 2835519
  3. Six distinct nuclear factors interact with the 75-base-pair repeat of the Moloney murine leukemia virus enhancer.
    Mol Cell Biol. 1987 Mar;7(3):1101-10 PMID: 3561410
  4. Structural diversity and nuclear protein binding sites in the long terminal repeats of feline leukemia virus.
    J Virol. 1990 Apr;64(4):1675-82 PMID: 2157050
  5. Protein recognition sites in polyomavirus enhancer: formation of a novel site for NF-1 factor in an enhancer mutant and characterization of a site in the enhancer D domain.
    EMBO J. 1990 Mar;9(3):947-55 PMID: 2155786
  6. Alignment of U3 region sequences of mammalian type C viruses: identification of highly conserved motifs and implications for enhancer design.
    J Virol. 1990 Feb;64(2):534-42 PMID: 2153223
  7. Differential expression of jun and fos genes during differentiation of mouse P19 embryonal carcinoma cells.
    Nucleic Acids Res. 1990 Jun 11;18(11):3195-202 PMID: 2113273
  8. Chicken NFI/TGGCA proteins are encoded by at least three independent genes: NFI-A, NFI-B and NFI-C with homologues in mammalian genomes.
    Nucleic Acids Res. 1990 May 11;18(9):2607-16 PMID: 2339052
  9. Analysis of multiple forms of nuclear factor I in human and murine cell lines.
    Mol Cell Biol. 1990 Mar;10(3):1041-8 PMID: 2304457
  10. Identification and purification of EBP1: a HeLa cell protein that binds to a region overlapping the 'core' of the SV40 enhancer.
    Genes Dev. 1988 Aug;2(8):991-1002 PMID: 2844627
  11. Nucleotide sequences of a feline leukemia virus subgroup A envelope gene and long terminal repeat and evidence for the recombinational origin of subgroup B viruses.
    J Virol. 1986 Jun;58(3):825-34 PMID: 3009890
  12. Correlation of leukemogenic potential of murine retroviruses with transcriptional tissue preference of the viral long terminal repeats.
    J Virol. 1987 Apr;61(4):1067-72 PMID: 3029400
  13. Induction of a factor that binds to the polyoma virus A enhancer on differentiation of embryonal carcinoma cells.
    Nature. 1987 Jul 16-22;328(6127):254-6 PMID: 3037385
  14. Activation of DNA-binding activity in an apparently cytoplasmic precursor of the NF-kappa B transcription factor.
    Cell. 1988 Apr 22;53(2):211-7 PMID: 3129195
  15. Purification of a NF1-like DNA-binding protein from rat liver and cloning of the corresponding cDNA.
    EMBO J. 1988 Oct;7(10):3115-23 PMID: 3053160
  16. Trans-acting protein factors and the regulation of eukaryotic transcription: lessons from studies on DNA tumor viruses.
    Genes Dev. 1988 Mar;2(3):267-81 PMID: 3288540
  17. In vitro activation and nuclear translocation of NF-kappa B catalyzed by cyclic AMP-dependent protein kinase and protein kinase C.
    Mol Cell Biol. 1989 Jun;9(6):2424-30 PMID: 2548081
  18. Mutant analysis of protein interactions with a nuclear factor I binding site in the SL3-3 virus enhancer.
    Nucleic Acids Res. 1989 Jun 12;17(11):4061-75 PMID: 2544855
  19. Identification of the SL3-3 virus enhancer core as a T-lymphoma cell-specific element.
    J Virol. 1989 Jan;63(1):76-84 PMID: 2535754
  20. The proline-rich transcriptional activator of CTF/NF-I is distinct from the replication and DNA binding domain.
    Cell. 1989 Aug 25;58(4):741-53 PMID: 2504497
  21. Eukaryotic transcriptional regulatory proteins.
    Annu Rev Biochem. 1989;58:799-839 PMID: 2673023
  22. Regulated phosphorylation and dephosphorylation of GAL4, a transcriptional activator.
    Genes Dev. 1989 Aug;3(8):1157-65 PMID: 2676720
  23. Differences in activities of murine retroviral long terminal repeats in cytotoxic T lymphocytes and T-lymphoma cells.
    J Virol. 1989 Mar;63(3):1087-94 PMID: 2644446
  24. Cyclic AMP-dependent protein kinase phosphorylates and inactivates the yeast transcriptional activator ADR1.
    Cell. 1989 Feb 10;56(3):409-19 PMID: 2644045
  25. Multiple genes encode nuclear factor 1-like proteins that bind to the promoter for 3-hydroxy-3-methylglutaryl-coenzyme A reductase.
    Proc Natl Acad Sci U S A. 1988 Dec;85(23):8963-7 PMID: 3194401
  26. A family of human CCAAT-box-binding proteins active in transcription and DNA replication: cloning and expression of multiple cDNAs.
    Nature. 1988 Jul 21;334(6179):218-24 PMID: 3398920
  27. The pIC plasmid and phage vectors with versatile cloning sites for recombinant selection by insertional inactivation.
    Gene. 1984 Dec;32(3):481-5 PMID: 6099327
  28. Mapping the viral sequences conferring leukemogenicity and disease specificity in Moloney and amphotropic murine leukemia viruses.
    J Virol. 1984 Nov;52(2):448-56 PMID: 6092670
  29. Rapid and efficient site-specific mutagenesis without phenotypic selection.
    Methods Enzymol. 1987;154:367-82 PMID: 3323813
  30. Role for the 3' end of the genome in determining disease specificity of Friend and Moloney murine leukemia viruses.
    Proc Natl Acad Sci U S A. 1983 Jul;80(14):4408-11 PMID: 6308622
  31. Transduction and rearrangement of the myc gene by feline leukaemia virus in naturally occurring T-cell leukaemias.
    Nature. 1984 Apr 26-May 2;308(5962):814-20 PMID: 6325920
  32. Expression and regulation of Escherichia coli lacZ gene fusions in mammalian cells.
    J Mol Appl Genet. 1983;2(1):101-9 PMID: 6302193
  33. Multiple arrangements of viral DNA and an activated host oncogene in bursal lymphomas.
    Nature. 1982 Jan 21;295(5846):209-14 PMID: 6276760
  34. Activation of a cellular onc gene by promoter insertion in ALV-induced lymphoid leukosis.
    Nature. 1981 Apr 9;290(5806):475-80 PMID: 6261142
  35. Recombinants between endogenous and exogenous avian tumor viruses: role of the C region and other portions of the genome in the control of replication and transformation.
    J Virol. 1980 Jan;33(1):238-49 PMID: 6245229
  36. Specific binding of a cellular DNA replication protein to the origin of replication of adenovirus DNA.
    Proc Natl Acad Sci U S A. 1983 Oct;80(20):6177-81 PMID: 6336326
  37. Characterisation of a tumour-specific antigen on the surface of feline lymphosarcoma cells.
    Nature. 1978 Oct 19;275(5681):656-8 PMID: 212681
  38. Alkaline phosphatase activity in mouse teratoma.
    Proc Natl Acad Sci U S A. 1973 Dec;70(12):3899-903 PMID: 4521215
  39. "C"-type viral particles in plasma of cats with feline leukemia.
    Science. 1967 Nov;158(3804):1049-50 PMID: 6058344
  40. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  41. Activation in vitro of sequence-specific DNA binding by a human regulatory factor.
    Nature. 1988 Sep 22;335(6188):372-5 PMID: 3419505
  42. GATAAG; a cis-control region binding an erythroid-specific nuclear factor with a role in globin and non-globin gene expression.
    Nucleic Acids Res. 1989 Jan 11;17(1):73-92 PMID: 2911489
  43. Isolation of a recombinant copy of the gene encoding C/EBP.
    Genes Dev. 1988 Jul;2(7):786-800 PMID: 2850264
  44. A cellular DNA-binding protein that activates eukaryotic transcription and DNA replication.
    Cell. 1987 Jan 16;48(1):79-89 PMID: 3024847
  45. Phosphorylation-induced binding and transcriptional efficacy of nuclear factor CREB.
    Nature. 1988 Aug 11;334(6182):494-8 PMID: 2900470
  46. Retroviral transduction of T-cell antigen receptor beta-chain and myc genes.
    Nature. 1987 Mar 12-18;326(6109):190-4 PMID: 3029597
  47. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
    Anal Biochem. 1987 Apr;162(1):156-9 PMID: 2440339
  48. Human growth hormone as a reporter gene in regulation studies employing transient gene expression.
    Mol Cell Biol. 1986 Sep;6(9):3173-9 PMID: 3023965
  49. A nuclear factor 1 binding site mediates the transcriptional activation of a type I collagen promoter by transforming growth factor-beta.
    Cell. 1988 Feb 12;52(3):405-14 PMID: 2830985
  50. Differential protein binding in lymphocytes to a sequence in the enhancer of the mouse retrovirus SL3-3.
    Mol Cell Biol. 1988 Apr;8(4):1625-37 PMID: 2837650
  51. Heat-inducible human factor that binds to a human hsp70 promoter.
    Mol Cell Biol. 1987 Apr;7(4):1530-4 PMID: 3600634
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.65.4.1991-1999.1991
Published
1991-04-00
Pages
1991-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC240038
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.65.4.1991-1999.1991 PMC Full Text View journal details
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