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PMID: 1849280 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Expression of three mammalian cDNAs that interfere with RAS function in Saccharomyces cerevisiae.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 7 ·1991-04-01 ·Pages 2913-7

Colicelli J, Nicolette C, Birchmeier C, Rodgers L, Riggs M, Wigler M

Abstract

Saccharomyces cerevisiae strains expressing the activated RAS2Val19 gene or lacking both cAMP phosphodiesterase genes, PDE1 and PDE2, have impaired growth control and display an acute sensitivity to heat shock. We have isolated two classes of mammalian cDNAs from yeast expression libraries that suppress the heat shock-sensitive phenotype of RAS2Val19 strain. Members of the first class of cDNAs also suppress the heat shock-sensitive phenotype of pde1- pde2- strains and encode cAMP phosphodiesterases. Members of the second class fail to suppress the phenotype of pde1- pde2- strains and therefore are candidate cDNAs encoding proteins that interact with RAS proteins. We report the nucleotide sequence of three members of this class. Two of these cDNAs share considerable sequence similarity, but none are clearly similar to previously isolated genes.

MeSH Terms
3',5'-Cyclic-AMP Phosphodiesterases/genetics Amino Acid Sequence Base Sequence Cloning, Molecular Cyclic Nucleotide Phosphodiesterases, Type 1 DNA/genetics Escherichia coli/genetics Fungal Proteins/genetics GTP-Binding Proteins/genetics Gene Library Genes, Fungal Genes, Suppressor Genes, ras Humans Molecular Sequence Data Oligonucleotide Probes Plasmids Restriction Mapping Saccharomyces cerevisiae/genetics Sequence Homology, Nucleic Acid ras Proteins
Chemicals
Fungal Proteins Oligonucleotide Probes DNA 3',5'-Cyclic-AMP Phosphodiesterases Cyclic Nucleotide Phosphodiesterases, Type 1 GTP-Binding Proteins ras Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Colicelli J
Cold Spring Harbor Laboratory, NY 11724.
Nicolette C
Birchmeier C
Rodgers L
Riggs M
Wigler M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.7.2913
Published
1991-04-01
Pages
2913-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC51350
Subset
IM
Databases
GENBANK
D90292, D90293, D90294, D90295, D90296, D90297, D90298, M37190, M37191, M37192
External Links
PubMed source DOI: 10.1073/pnas.88.7.2913 PMC Full Text View journal details
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