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PMID: 18511435 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Genome structure of the legume, Lotus japonicus.

DNA research : an international journal for rapid publication of reports on genes and genomes ·Vol. 15 ·No. 4 ·2008-08-00 ·Pages 227-39

Sato S, Nakamura Y, Kaneko T, Asamizu E, Kato T, Nakao M, Sasamoto S, Watanabe A, Ono A, Kawashima K, Fujishiro T, Katoh M, Kohara M, Kishida Y, Minami C, Nakayama S, Nakazaki N, Shimizu Y, Shinpo S, Takahashi C, Wada T, Yamada M, Ohmido N, Hayashi M, Fukui K, Baba T, Nakamichi T, Mori H, Tabata S

Abstract

The legume Lotus japonicus has been widely used as a model system to investigate the genetic background of legume-specific phenomena such as symbiotic nitrogen fixation. Here, we report structural features of the L. japonicus genome. The 315.1-Mb sequences determined in this and previous studies correspond to 67% of the genome (472 Mb), and are likely to cover 91.3% of the gene space. Linkage mapping anchored 130-Mb sequences onto the six linkage groups. A total of 10,951 complete and 19,848 partial structures of protein-encoding genes were assigned to the genome. Comparative analysis of these genes revealed the expansion of several functional domains and gene families that are characteristic of L. japonicus. Synteny analysis detected traces of whole-genome duplication and the presence of synteny blocks with other plant genomes to various degrees. This study provides the first opportunity to look into the complex and unique genetic system of legumes.

MeSH Terms
Chromosome Mapping DNA, Plant Gene Duplication Genes, Plant Genome, Plant In Situ Hybridization, Fluorescence Lotus/genetics Plant Proteins/genetics,metabolism Repetitive Sequences, Nucleic Acid Sequence Analysis, DNA Synteny
Chemicals
DNA, Plant Plant Proteins
Authors & Affiliations
29 authors, click to expand affiliations / ORCID
Sato Shusei
Kazusa DNA Research Institute, 2-6-7 Kazusa-kamatari, Kisarazu, Chiba, Japan.
Nakamura Yasukazu
Kaneko Takakazu
Asamizu Erika
Kato Tomohiko
Nakao Mitsuteru
Sasamoto Shigemi
Watanabe Akiko
Ono Akiko
Kawashima Kumiko
Fujishiro Tsunakazu
Katoh Midori
Kohara Mitsuyo
Kishida Yoshie
Minami Chiharu
Nakayama Shinobu
Nakazaki Naomi
Shimizu Yoshimi
Shinpo Sayaka
Takahashi Chika
Wada Tsuyuko
Yamada Manabu
Ohmido Nobuko
Hayashi Makoto
Fukui Kiichi
Baba Tomoya
Nakamichi Tomoko
Mori Hirotada
Tabata Satoshi
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Article Info
Journal
DNA research : an international journal for rapid publication of reports on genes and genomes
Abbr.
DNA Res
ISSN
1756-1663
DOI
10.1093/dnares/dsn008
Published
2008-08-00
Epub
2008-00-28
Pages
227-39
Language
English
Region
England
NLM ID
9423827
PMCID
PMC2575887
Subset
IM
External Links
PubMed source DOI: 10.1093/dnares/dsn008 PMC Full Text View journal details
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