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PMID: 18515193 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

The histone methylase Set2p and the histone deacetylase Rpd3p repress meiotic recombination at the HIS4 meiotic recombination hotspot in Saccharomyces cerevisiae.

DNA repair ·Vol. 7 ·No. 8 ·2008-08-02 ·Pages 1298-308

Merker JD, Dominska M, Greenwell PW, Rinella E, Bouck DC, Shibata Y, Strahl BD, Mieczkowski P, Petes TD

Abstract

The rate of meiotic recombination in the yeast Saccharomyces cerevisiae varies widely in different regions of the genome with some genes having very high levels of recombination (hotspots). A variety of experiments done in yeast suggest that hotspots are a feature of chromatin structure rather than a feature of primary DNA sequence. We examined the effects of mutating a variety of enzymes that affect chromatin structure on the recombination activity of the well-characterized HIS4 hotspot including the Set2p and Dot1p histone methylases, the Hda1p and Rpd3p histone deacetylases, the Sin4p global transcription regulator, and a deletion of one of the two copies of the genes encoding histone H3-H4. Loss of Set2p or Rpd3p substantially elevated HIS4 hotspot activity, and loss of Hda1p had a smaller stimulatory effect; none of the other alterations had a significant effect. The increase of HIS4 hotspot activity in set2 and rpd3 strains is likely to be related to the recent finding that histone H3 methylation by Set2p directs deacetylation of histones by Rpd3p.

MeSH Terms
Alcohol Oxidoreductases/genetics Aminohydrolases/genetics Base Sequence Chromatin Immunoprecipitation DNA Primers Histone Deacetylases/physiology Histones/physiology Meiosis/physiology Methyltransferases/physiology Pyrophosphatases/genetics Recombination, Genetic/physiology Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins/genetics,physiology
Chemicals
DNA Primers Histones Saccharomyces cerevisiae Proteins Alcohol Oxidoreductases HIS4 protein, S cerevisiae Methyltransferases Set2 protein, S cerevisiae RPD3 protein, S cerevisiae Histone Deacetylases Aminohydrolases Pyrophosphatases
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Merker Jason D
Department of Biology, University of North Carolina, Chapel Hill, NC 27599-3280, USA.
Dominska Margaret
Greenwell Patricia W
Rinella Erica
Bouck David C
Shibata Yoichiro
Strahl Brian D
Mieczkowski Piotr
Petes Thomas D
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Article Info
Journal
DNA repair
Abbr.
DNA Repair (Amst)
ISSN
1568-7864
DOI
10.1016/j.dnarep.2008.04.009
Published
2008-08-02
Epub
2008-00-02
Pages
1298-308
Language
English
Region
Netherlands
NLM ID
101139138
PMCID
PMC2518087
Subset
IM
Grants
NIGMS NIH HHS · R01 GM068088 · United States
NIGMS NIH HHS · GM 68088 · United States
NIGMS NIH HHS · R01 GM024110 · United States
NIGMS NIH HHS · R01 GM024110-31 · United States
NIGMS NIH HHS · GM 24110 · United States
External Links
PubMed source DOI: 10.1016/j.dnarep.2008.04.009 PMC Full Text View journal details
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