Home LiteratureArticle Details
PMID: 18516087 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Review

T-cell antigen receptor triggering and lipid rafts: a matter of space and time scales. Talking Point on the involvement of lipid rafts in T-cell activation.

EMBO reports ·Vol. 9 ·No. 6 ·2008-06-00 ·Pages 525-30

He HT, Marguet D

Abstract

T-cell antigen receptor triggering mechanisms and lipid rafts are of broad interest, but are also controversial topics. Here, we review some recent progress in these two research fields, which has been accomplished mostly in live cells and with the use of advanced technologies. We then discuss the potential relationship between membrane-domain organization and T-cell antigen receptor-triggering mechanisms. On the basis of the relevant experimental observations, we argue that the key to achieving a better understanding of both processes is the ability to monitor the molecular dynamics and interactions taking place in the membrane of T cells at a spatial scale of tens to hundreds of nanometres, with a subsecond-to-second temporal resolution.

MeSH Terms
Diffusion Humans Lymphocyte Activation Membrane Lipids/chemistry,metabolism Membrane Microdomains/chemistry,metabolism Receptors, Antigen, T-Cell/metabolism Signal Transduction/physiology T-Lymphocytes/cytology,physiology Time Factors
Chemicals
Membrane Lipids Receptors, Antigen, T-Cell
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
He Hai-Tao
Centre d'Immunologie de Marseille-Luminy, Case 906, F13288 Marseille Cedex 09, France. [email protected]
Marguet Didier
References (55)
55 references, click to expand
  1. Paradigm shift of the plasma membrane concept from the two-dimensional continuum fluid to the partitioned fluid: high-speed single-molecule tracking of membrane molecules.
    Annu Rev Biophys Biomol Struct. 2005;34:351-78 PMID: 15869394
  2. Plasma membrane nanoswitches generate high-fidelity Ras signal transduction.
    Nat Cell Biol. 2007 Aug;9(8):905-14 PMID: 17618274
  3. Dynamic molecular confinement in the plasma membrane by microdomains and the cytoskeleton meshwork.
    EMBO J. 2006 Jul 26;25(14):3245-56 PMID: 16858413
  4. Palmitoylation is required for efficient Fas cell death signaling.
    EMBO J. 2007 Jan 10;26(1):209-20 PMID: 17159908
  5. An evolutionary and structural perspective on T cell antigen receptor function.
    Immunol Rev. 2003 Feb;191:7-27 PMID: 12614348
  6. S-acylation of LCK protein tyrosine kinase is essential for its signalling function in T lymphocytes.
    EMBO J. 1997 Aug 15;16(16):4983-98 PMID: 9305640
  7. Structure and origin of ordered lipid domains in biological membranes.
    J Membr Biol. 1998 Jul 15;164(2):103-14 PMID: 9662555
  8. Probing T cell membrane organization using dimeric MHC-Ig complexes.
    J Immunol Methods. 2002 Oct 1;268(1):93-106 PMID: 12213346
  9. Condensation of the plasma membrane at the site of T lymphocyte activation.
    J Cell Biol. 2005 Oct 10;171(1):121-31 PMID: 16203859
  10. Visualizing lipid structure and raft domains in living cells with two-photon microscopy.
    Proc Natl Acad Sci U S A. 2003 Dec 23;100(26):15554-9 PMID: 14673117
  11. Actin and agonist MHC-peptide complex-dependent T cell receptor microclusters as scaffolds for signaling.
    J Exp Med. 2005 Oct 17;202(8):1031-6 PMID: 16216891
  12. Kinetics of MHC-CD8 interaction at the T cell membrane.
    J Immunol. 2007 Dec 1;179(11):7653-62 PMID: 18025211
  13. The oxygen-substituted palmitic acid analogue, 13-oxypalmitic acid, inhibits Lck localization to lipid rafts and T cell signaling.
    Biochim Biophys Acta. 2002 Apr 3;1589(2):140-50 PMID: 12007789
  14. Characterization of membrane domains by FRAP experiments at variable observation areas.
    Eur Biophys J. 1998;27(4):391-402 PMID: 9691468
  15. Fanciful FRET.
    Sci STKE. 2006 Apr 18;2006(331):re2 PMID: 16622184
  16. Spatial and temporal dynamics of T cell receptor signaling with a photoactivatable agonist.
    Immunity. 2007 Jul;27(1):76-88 PMID: 17629516
  17. Fluorescence correlation spectroscopy diffusion laws to probe the submicron cell membrane organization.
    Biophys J. 2005 Dec;89(6):4029-42 PMID: 16199500
  18. Lipid raft proteins have a random distribution during localized activation of the T-cell receptor.
    Nat Cell Biol. 2004 Mar;6(3):238-43 PMID: 14767481
  19. Newly generated T cell receptor microclusters initiate and sustain T cell activation by recruitment of Zap70 and SLP-76.
    Nat Immunol. 2005 Dec;6(12):1253-62 PMID: 16273097
  20. Phospholipids undergo hop diffusion in compartmentalized cell membrane.
    J Cell Biol. 2002 Jun 10;157(6):1071-81 PMID: 12058021
  21. TCR signal initiation machinery is pre-assembled and activated in a subset of membrane rafts.
    EMBO J. 2002 Apr 15;21(8):1899-908 PMID: 11953309
  22. T cell receptor-proximal signals are sustained in peripheral microclusters and terminated in the central supramolecular activation cluster.
    Immunity. 2006 Jul;25(1):117-27 PMID: 16860761
  23. Micrometer-scale domains in fibroblast plasma membranes.
    J Cell Biol. 1987 Aug;105(2):755-60 PMID: 3624308
  24. Activated K-Ras and H-Ras display different interactions with saturable nonraft sites at the surface of live cells.
    J Cell Biol. 2002 May 27;157(5):865-72 PMID: 12021258
  25. Quantification and correction of systematic errors due to detector time-averaging in single-molecule tracking experiments.
    Biophys J. 2006 Jan 15;90(2):L17-9 PMID: 16299068
  26. FRET imaging reveals that functional neurokinin-1 receptors are monomeric and reside in membrane microdomains of live cells.
    Proc Natl Acad Sci U S A. 2006 Feb 14;103(7):2138-43 PMID: 16461466
  27. The kinetic-segregation model: TCR triggering and beyond.
    Nat Immunol. 2006 Aug;7(8):803-9 PMID: 16855606
  28. Coexistence of multivalent and monovalent TCRs explains high sensitivity and wide range of response.
    J Exp Med. 2005 Aug 15;202(4):493-503 PMID: 16087711
  29. The state of lipid rafts: from model membranes to cells.
    Annu Rev Biophys Biomol Struct. 2003;32:257-83 PMID: 12543707
  30. Dynamics in the plasma membrane: how to combine fluidity and order.
    EMBO J. 2006 Aug 9;25(15):3446-57 PMID: 16900097
  31. Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells.
    Science. 2002 May 3;296(5569):913-6 PMID: 11988576
  32. Nanoscale organization of multiple GPI-anchored proteins in living cell membranes.
    Cell. 2004 Feb 20;116(4):577-89 PMID: 14980224
  33. CD8beta endows CD8 with efficient coreceptor function by coupling T cell receptor/CD3 to raft-associated CD8/p56(lck) complexes.
    J Exp Med. 2001 Nov 19;194(10):1485-95 PMID: 11714755
  34. CD3 delta establishes a functional link between the T cell receptor and CD8.
    J Biol Chem. 2003 Jan 31;278(5):3257-64 PMID: 12215456
  35. Direct visualization of Ras proteins in spatially distinct cell surface microdomains.
    J Cell Biol. 2003 Jan 20;160(2):165-70 PMID: 12527752
  36. Agonist/endogenous peptide-MHC heterodimers drive T cell activation and sensitivity.
    Nature. 2005 Mar 10;434(7030):238-43 PMID: 15724150
  37. Essential role of CD8 palmitoylation in CD8 coreceptor function.
    J Immunol. 2000 Aug 15;165(4):2068-76 PMID: 10925291
  38. Lipid raft distribution of CD4 depends on its palmitoylation and association with Lck, and evidence for CD4-induced lipid raft aggregation as an additional mechanism to enhance CD3 signaling.
    J Immunol. 2003 Jan 15;170(2):913-21 PMID: 12517957
  39. The immunological synapse balances T cell receptor signaling and degradation.
    Science. 2003 Nov 14;302(5648):1218-22 PMID: 14512504
  40. Ligand recognition by alpha beta T cell receptors.
    Annu Rev Immunol. 1998;16:523-44 PMID: 9597140
  41. Phosphorylation of T cell receptor zeta is regulated by a lipid dependent folding transition.
    Nat Struct Biol. 2000 Nov;7(11):1023-6 PMID: 11062556
  42. Single-molecule microscopy reveals plasma membrane microdomains created by protein-protein networks that exclude or trap signaling molecules in T cells.
    Cell. 2005 Jun 17;121(6):937-50 PMID: 15960980
  43. Lipid rafts and the initiation of T cell receptor signaling.
    Semin Immunol. 2005 Feb;17(1):23-33 PMID: 15582486
  44. The dynamics of T cell receptor signaling: complex orchestration and the key roles of tempo and cooperation.
    Annu Rev Immunol. 1999;17:467-522 PMID: 10358766
  45. Altered TCR signaling from geometrically repatterned immunological synapses.
    Science. 2005 Nov 18;310(5751):1191-3 PMID: 16293763
  46. Single-molecule mountains yield nanoscale cell images.
    Nat Methods. 2006 Oct;3(10):781-2 PMID: 16990808
  47. (Un)confined diffusion of CD59 in the plasma membrane determined by high-resolution single molecule microscopy.
    Biophys J. 2007 May 15;92(10):3719-28 PMID: 17325009
  48. Macromolecular-scale resolution in biological fluorescence microscopy.
    Proc Natl Acad Sci U S A. 2006 Aug 1;103(31):11440-5 PMID: 16864773
  49. Signal transduction by lymphocyte antigen receptors.
    Cell. 1994 Jan 28;76(2):263-74 PMID: 8293463
  50. Increased TCR avidity after T cell activation: a mechanism for sensing low-density antigen.
    Immunity. 2001 Feb;14(2):135-43 PMID: 11239446
  51. CD28 interaction with filamin-A controls lipid raft accumulation at the T-cell immunological synapse.
    Nat Cell Biol. 2006 Nov;8(11):1270-6 PMID: 17060905
  52. Distribution of a glycosylphosphatidylinositol-anchored protein at the apical surface of MDCK cells examined at a resolution of <100 A using imaging fluorescence resonance energy transfer.
    J Cell Biol. 1998 Jul 13;142(1):69-84 PMID: 9660864
  53. Functional rafts in cell membranes.
    Nature. 1997 Jun 5;387(6633):569-72 PMID: 9177342
  54. Diffusion analysis within single nanometric apertures reveals the ultrafine cell membrane organization.
    Biophys J. 2007 Feb 1;92(3):913-9 PMID: 17085499
  55. Gangliosides GM1 and GM3 in the living cell membrane form clusters susceptible to cholesterol depletion and chilling.
    Mol Biol Cell. 2007 Jun;18(6):2112-22 PMID: 17392511
Article Info
Journal
EMBO reports
Abbr.
EMBO Rep
ISSN
1469-3178
DOI
10.1038/embor.2008.78
Email
[email protected]
Published
2008-06-00
Pages
525-30
Language
English
Region
England
NLM ID
100963049
PMCID
PMC2427382
Subset
IM
Corrections
CommentIn
PMID: 18516086
External Links
PubMed source DOI: 10.1038/embor.2008.78 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2